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Schuurman, J.L.* ; van Tetering, L.* ; Houthuijs, K.J.* ; Kooijman, P.* ; Gailus-Durner, V. ; Leuchtenberger, S. ; Fuchs, H. ; Hrabě de Angelis, M. ; Engelke, U.F.H.* ; Lefeber, D.J.* ; van Karnebeek, C.D.M.* ; Wevers, R.A.* ; Grgic, A.* ; Balluf, B.* ; Vandenbosch, M.* ; Vreeken, R.* ; Heeren, R.M.A.* ; Berden, G.* ; Oomens, J.* ; Martens, J.W.M.*

Structure elucidation for MALDI mass spectrometry imaging using infrared ion spectroscopy.

Anal. Chem., DOI: 10.1021/acs.analchem.5c00948 (2025)
Publ. Version/Full Text Research data DOI PMC
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Infrared ion spectroscopy (IRIS) is a tandem mass spectrometry (MS) technique that generates structurally diagnostic vibrational spectra for mass-selected ions trapped in a mass spectrometer. Until now, IRIS applications for biological samples have primarily focused on solution-based analyses, such as body fluids (e.g., plasma and urine) and tissue homogenates, using electrospray ionization (ESI) coupled with liquid chromatography-mass spectrometry (LC-MS). In this study, we have combined matrix-assisted laser desorption/ionization (MALDI) with IRIS for the direct analysis of small molecules from biological tissues on a Fourier-transform ion cyclotron resonance mass spectrometer. We applied this technique alongside MALDI mass spectrometry imaging to analyse brain tissue from two knockout mouse models of l-lysine catabolism disorders: pyridoxine-dependent epilepsy (ALDH7A1) and glutaric aciduria type 1 (GCDH). The MALDI-IRIS platform, now available for users at HFML-FELIX, represents a significant advance in the direct structural characterization of metabolites in complex biological tissues and opens new possibilities for structure elucidation in the field of MALDI mass spectrometry imaging.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Pyridoxine-dependent Epilepsy; Ultraviolet-laser Desorption; Mouse Model; Brain; Ionization; Metabolism; Biomarkers; Antiquitin
Language english
Publication Year 2025
HGF-reported in Year 2025
ISSN (print) / ISBN 0003-2700
e-ISSN 1520-6882
Journal Analytical Chemistry
Publisher American Chemical Society (ACS)
Publishing Place 1155 16th St, Nw, Washington, Dc 20036 Usa
Reviewing status Peer reviewed
Institute(s) Institute of Experimental Genetics (IEG)
POF-Topic(s) 30201 - Metabolic Health
Research field(s) Genetics and Epidemiology
PSP Element(s) G-500600-001
Grants NWO Rekentijd grant
Dutch Research Council (NWO)
Changing Rare Disorders of Lysine Metabolism (CHARLIE) consortium
DOI 10.1021/acs.analchem.5c00948
WOS ID 001510174800001
Scopus ID 105009107937
PubMed ID 40521743
Erfassungsdatum 2025-06-23
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