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Open Access Green as soon as Postprint is submitted to ZB.
ORF23 of murine gammaherpesvirus 68 is non-essential for in vitro and in vivo infection.
J. Gen. Virol. 93, 1076-1080 (2012)
Although ORF23 is conserved among gammaherpesviruses, its role during infection is unknown. Here, we studied the expression of ORF23 of murine gammaherpesvirus 68 (MHV-68) and its role during infection. ORF23 mRNA was detected in infected cells as a late transcript. The ORF23 protein product could be expressed and detected as an N-terminally FLAG-tagged protein by Western blot and indirect immunofluorescence. To investigate the role of ORF23 in the infection cycle of a gammaherpesvirus, we constructed an ORF23 deletion mutant of MHV-68. The analysis of the ORF23 deletion mutant suggested that ORF23 of MHV-68 is neither essential for replication in cell culture nor for lytic or latent infection in vivo. A phenotype of the ORF23 deletion mutant, reflected by a moderate reduction in lytic replication and latency amplification, was only detectable in the face of direct competition to the parental virus.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
BACTERIAL ARTIFICIAL CHROMOSOME; MOUSE MODEL; HERPESVIRUS; PROTEINS; VIRUS; IDENTIFICATION; PATHOGENESIS; MUTAGENESIS; EXPRESSION; GENETICS
ISSN (print) / ISBN
0022-1317
e-ISSN
1465-2099
Journal
Journal of General Virology
Quellenangaben
Volume: 93,
Issue: Part 5,
Pages: 1076-1080
Publisher
Society for General Microbiology
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Research Unit Gene Vector (AGV)