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Vonficht, D.* ; Jopp-Saile, L.* ; Yousefian, S.* ; Flore, V.* ; Simó Vesperinas, I.* ; Teuber, R.* ; Avanesyan, B.* ; Luo, Y.* ; Röthemeier, C.* ; Grünschläger, F.* ; Fernández-Vaquero, M.* ; Fregona, V.* ; Ordoñez-Rueda, D.* ; Schmalbrock, L.K.* ; Deininger, L.* ; Yamachui Sitcheu, A.J.* ; Gu, Z.* ; Funk, M.C. ; Mikut, R.* ; Heikenwälder, M.* ; Eggert, A.* ; von Stackelberg, A.* ; Kobold, S. ; Krönke, J.* ; Keller, U.* ; Trumpp, A.* ; Hegazy, A.N.* ; Eckert, C.* ; Hübschmann, D.* ; Haas, S.*

Ultra-high-scale cytometry-based cellular interaction mapping.

Nat. Methods 22, 1887–1899 (2025)
Publ. Version/Full Text Research data DOI PMC
Open Access Hybrid
Creative Commons Lizenzvertrag
Cellular interactions are of fundamental importance, orchestrating organismal development, tissue homeostasis and immunity. Recently, powerful methods that use single-cell genomic technologies to dissect physically interacting cells have been developed. However, these approaches are characterized by low cellular throughput, long processing times and high costs and are typically restricted to predefined cell types. Here we introduce Interact-omics, a cytometry-based framework to accurately map cellular landscapes and cellular interactions across all immune cell types at ultra-high resolution and scale. We demonstrate the utility of our approach to study kinetics, mode of action and personalized response prediction of immunotherapies, and organism-wide shifts in cellular composition and cellular interaction dynamics following infection in vivo. Our scalable framework can be applied a posteriori to existing cytometry datasets or incorporated into newly designed cytometry-based studies to map cellular interactions with a broad range of applications from fundamental biology to applied biomedicine.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Bone-marrow; T-cells; Transgenic Mice; Flow; Identification; Visualization; Blinatumomab; Expression; Algorithm; Responses
ISSN (print) / ISBN 1548-7091
e-ISSN 1548-7105
Journal Nature Methods
Quellenangaben Volume: 22, Issue: , Pages: 1887–1899 Article Number: , Supplement: ,
Publisher Nature Publishing Group
Publishing Place New York, NY
Reviewing status Peer reviewed
Institute(s) Institute of Lung Health and Immunity (LHI)
Unit for Clinical Pharmacology (KKG-EKLiP)
Grants European Union (ERC-StG, InteractOmics)
Berlin Institute of Health
Dietmar Hopp Foundation
Lichtenberg fellowship
Helmholtz Association
Volkswagen Foundation
Deutsches Krebsforschungszentrum (DKFZ)
BIH Clinician Scientist grant
German Research Foundation grant
ERC Advanced Grant SHATTER-AML
Charite - Universitaetsmedizin Berlin
Molecular Precision Oncology Program (MPOP) of the National Center for Tumor Diseases (NCT) Heidelberg
ERC-PoC, Interact-ALL
Heisenberg program of the German Research Foundation (DFG)
The e:Med LeukoSyStem consortium (BMBF)
HEROES-AYA consortium (BMBF)
TEP-CC consortium (Bruno and Helene Joester Foundation)
DFG
Joachim Herz Stiftung
ERC-StG 'iMOTIONS' grant
European Research Council
SFB-TRR grant
Fritz-Bender Foundation
Deutsche Jose Carreras Leukaemie Stiftung
Hector Foundation
Bavarian Research Foundation (BAYCELLATOR)
Bruno and Helene Joester Foundation (360degrees CAR)
German Childhood Cancer Foundation
German Cancer Consortium (DKTK Call 2021-2023)
M4-Award of the Bavarian Ministry for Economical Affairs, Bundesministerium fur Bildung und Forschung (CONTRACT)
Bavarian Cancer Research Center (BZKF) (TANGO)
Deutsche Forschungsgemeinschaft (DFG)
Elite Network of Bavaria
Horizon 2020 programme of the European Union
Else Kroener-Fresenius-Stiftung (IOLIN)
German Cancer Aid (AvantCAR.de.)
Wilhelm-Sander-Stiftung
Go-Bio-Initiative