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Generation of a Flattop-T2A-H2B-Venus x C-peptide-mCherry double reporter human iPSC line to monitor WNT/Planar cell polarity pathway activity.
Stem Cell Res. 88:103838 (2025)
Deriving functional β-cells from human induced pluripotent stem cells (hiPSCs) holds potential for cell replacement therapy, disease modeling, and drug testing in diabetes research. Wnt/Planar cell polarity (PCP) signaling is crucial for endocrine cell development and β-cell maturation in murine models and can be tracked by the expression of the tissue-specific effector gene Flattop. Here, we report the generation of a human fluorescent FLTP/CFAP126 (Flattop-T2A-H2B-Venus) and FLTP-Insulin (Flattop-T2A-H2B-Venus x C-peptide-mCherry) double reporter by CRISPR/Cas9 gene editing. These hiPSC reporter lines allow monitoring of WNT/PCP signaling during endocrine cell formation and studying its role in β-cells in a human model system.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
1873-5061
e-ISSN
1876-7753
Journal
Stem Cell Research
Quellenangaben
Volume: 88,
Article Number: 103838
Publisher
Elsevier
Publishing Place
Radarweg 29, 1043 Nx Amsterdam, Netherlands
Reviewing status
Peer reviewed
Grants
German Center for Diabetes Research (DZD e.V.)
Helmholtz-Gemeinschaft
EFSD and Lilly European Diabetes Research Programme
Helmholtz-Gemeinschaft
EFSD and Lilly European Diabetes Research Programme