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Kasemo, T.E.* ; Oppmann, M.* ; Dembski, S.* ; Steinke, M.* ; Lajtha, E.* ; Moratin, H.* ; Stöth, M.* ; Scherzad, A.* ; Delaval, M.N. ; Zimmermann, R. ; Di Bucchianico, S. ; Hackenberg, S.* ; Meyer, T.J.*

High concentrations of Printex 90 carbon black ultrafine particles disturb the epithelial barrier in human primary respiratory mucosa models.

Environ. Toxicol. Pharmacol. 119:104829 (2025)
Publ. Version/Full Text Research data DOI PMC
Open Access Hybrid
Creative Commons Lizenzvertrag
Airborne pollutants harm human health, but the mechanisms involved remain unclear. Impaired epithelial barrier function is, as in respiratory diseases, one possible pathomechanism. To investigate this, carbon black (CB) as a model for ultrafine particles, was applied to respiratory mucosa models of primary fibroblasts and epithelial cells cultured at the air-liquid interface (ALI). Models were assessed for the mucociliary phenotype. Cytotoxicity, DNA damage, and barrier integrity were evaluated by the lactate dehydrogenase (LDH) and comet assays, and by transepithelial electrical resistance (TEER) measurements. Cilia movement and ultrastructure, secretory cells, and intact cell-cell contacts were confirmed. Subtle changes were observed: the LDH release had increased 2h post exposure and barrier disturbance 24h post exposure was detected, both without mucosal damage or genotoxic effects. Donor-specific differences were present. Barrier disruption without cell detachment or death suggests model feasibility for long-term studies of, e.g., tissue regeneration or fibrosis following UFP exposure.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Carbon Black ; Air-liquid Interface ; Barrier ; Primary Cell Model ; Respiratory Mucosa ; Toxicology ; Ultrafine Particles; In-vitro; Genotoxicity; Nanotubes; Toxicity; Drug
ISSN (print) / ISBN 1382-6689
e-ISSN 1872-7077
Journal Environmental Toxicology and Pharmacology
Quellenangaben Volume: 119, Issue: , Pages: , Article Number: 104829 Supplement: ,
Publisher Elsevier
Publishing Place Radarweg 29, 1043 Nx Amsterdam, Netherlands
Reviewing status Peer reviewed
Institute(s) Cooperation Group Comprehensive Molecular Analytics (CMA)
Grants JEOL JEM 2100
Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)
Bavarian State Ministry of the Environment and Consumer Protection