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Open Access Green as soon as Postprint is submitted to ZB.
Directing neurotransmitter identity of neurones derived from expanded adult neural stem cells.
Eur. J. Neurosci. 25, 2581-2590 (2007)
In-vitro expanded neural stem cells (NSCs) of the adult subependymal zone (SEZ) may serve as a source for replacing degenerating neurones in disease and trauma. Crucial for the viability of this approach is the ability to selectively generate specific types of neurones from these cells. Here we show that NSCs derived from the adult mouse SEZ and expanded in vitro as neurosphere cells lose their in-vivo specification and generate a mixture of progeny comprising both GABAergic and also, surprisingly, glutamatergic neurones. When forced to express the pro-neural transcription factor neurogenin 2, virtually all progeny of in-vitro expanded adult NSCs acquire a glutamatergic identity, whereas only GABAergic neurones are generated upon expression of the transcription factor Mash1. Respecification of expanded NSCs from the adult SEZ by neurogenin 2 was accompanied by upregulation of the T-box transcription factor Tbr1, suggesting that their progeny had acquired a dorsal telencephalic identity. Thus, in-vitro expanded adult NSCs have the competence to become directed towards distinct functional neurotransmitter phenotypes when the appropriate transcriptional cues are provided.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
adult neurogenesis; cell fate; mouseneurogenic; repair; synapse formation
ISSN (print) / ISBN
0953-816X
e-ISSN
1460-9568
Journal
European Journal of Neuroscience
Quellenangaben
Volume: 25,
Issue: 9,
Pages: 2581-2590
Publisher
Wiley
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Stem Cell Research (ISF)