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Kukhtevich, I. ; Persson, S.* ; Padovani, F. ; Schneider, R. ; Cvijovic, M.* ; Schmoller, K.M.

The origin of septin ring size control in budding yeast.

EMBO J., DOI: 10.1038/s44318-025-00571-5 (2025)
Publ. Version/Full Text Research data DOI PMC
Open Access Hybrid
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The size of organelles and cellular structures needs to be tightly regulated and coordinated with overall cell size. A well-studied example is the Cdc42-driven polarization and subsequent septin ring formation in Saccharomyces cerevisiae, where the size of the resulting structures scales with cell size. However, the mechanisms underlying this scaling remain unclear. Here, we combine live-cell imaging, genetic perturbations, and three-dimensional mathematical modeling to investigate how septin ring size is controlled. Our integrative approach reveals that positive feedback in the polarization pathway, together with an increase of the amount of polarity proteins as cell size grows, can explain the scaling of the Cdc42 cluster and, consequently, septin ring diameter. Additionally, we show that in cells lacking the formin Bni1, where F-actin-cable assembly and directed polarization are disrupted, exocytosis becomes diffuse, leading to abnormally large septin rings. By integrating new experimental findings and mathematical modeling of yeast polarization, our study provides insights into the origin of septin ring size control.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Budding Yeast ; Cdc42 Polarization ; Cell Size ; Mechanistic Modeling ; Septin Ring; Negative Feedback; Cell Polarity; Cdc42p; Phosphorylation; Polarization; Dynamics; Formins; Driven
ISSN (print) / ISBN 0261-4189
e-ISSN 1460-2075
Journal EMBO Journal, The
Publisher Wiley
Publishing Place Heidelberg, Germany
Reviewing status Peer reviewed
Institute(s) Institute of Functional Epigenetics (IFE)
Grants Swedish Foundation for Strategic Research
Human Frontier Science Program
Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)
Helmholtz Gesellschaft

Swedish Research Council