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Ekino, S.* ; Arakawa, H. ; Sonoda, K.* ; Noguchi, K.* ; Inui, S.* ; Yokoyama, H.* ; Kodama, Y.*

The origin of IgG-containing cells in the bursa of Fabricius.

Cell Tissue Res. 348, 537-550 (2012)
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The bursa of Fabricius of the chicken is known as a primary lymphoid organ for B-cell development. Morphologically, the origin of IgG-containing cells in the bursa has not been clear until now, because abundant maternal IgG (MIgG) is transported to the chick embryo and distributed to the bursal tissue around hatching. Thus, it has been difficult to find out whether these cells themselves biosynthesize IgG or if they acquire MIgG via attachment to their surface. Our present study employing in situ hybridization clarified that IgG-containing cells in the medulla of bursal follicles did not biosynthesize IgG. To study the role of MIgG in the development of those IgG-containing cells, MIgG-free chicks were established from surgically bursectomized hen (SBx-hen). We found that, on the one hand, deprivation of MIgG from chicks completely inhibited the development of IgG-containing cells in the medulla after hatching. On the other hand, administration of MIgG to MIgG-free chicks recovered the emergence of those cells. In addition, we observed that those cells did not bear a B-cell marker and possessed dendrites with aggregated IgG. These results demonstrate that IgG-containing cells in the medulla are reticular cells that capture aggregated MIgG. Moreover, we show that the isolation of the bursa from environmental stimuli by bursal duct ligation (BDL) suppressed the development of IgG-containing cells after hatching. Thus, it is implied that environmental stimulations play a key role in MIgG aggregations and dendritic distributions of aggregated MIgG in the medulla after hatching.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Galt ; Igg-containing Cells ; Maternal Igg ; Reticular Cell ; The Bursa Of Fabricius; BINDING LECTIN MBL; ENVIRONMENTAL ANTIGENS; MONOCLONAL-ANTIBODIES; GENE CONVERSION; LYMPHOID-TISSUE; FC-RECEPTOR; CHICKEN; IMMUNOGLOBULIN; LOCALIZATION; FOLLICLES
ISSN (print) / ISBN 0044-3794
e-ISSN 1432-0878
Journal Cell and Tissue Research
Quellenangaben Volume: 348, Issue: 3, Pages: 537-550 Article Number: , Supplement: ,
Publisher Springer
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Translational Metabolic Oncology (TMO)