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HCS-3DX, a next-generation AI-driven automated 3D-oid high-content screening system.
Nat. Commun. 16:8897 (2025)
Self-organised three-dimensional (3D) cell cultures, collectively called 3D-oids, include spheroids, organoids and other co-culture models. Systematic evaluation of these models forms a critical new generation of high-content screening (HCS) systems for patient-specific drug analysis and cancer research. However, the standardisation of working with 3D-oids remains challenging and lacks convincing implementation. This study develops and tests HCS-3DX, a next-generation system for HCS analysis in 3D imaging and image evaluation. HCS-3DX is based on three main components: an automated Artificial Intelligence (AI)-driven micromanipulator for 3D-oid selection, an HCS foil multiwell plate for optimised imaging, and image-based AI software for single-cell data analysis. We validated HCS-3DX directly on 3D tumour models, including tumour-stroma co-cultures. Our data demonstrate that HCS-3DX achieves a resolution that overcomes the limitations of current systems and reliably and effectively performs 3D HCS at the single-cell level. Its application will enhance the accuracy and efficiency of drug screening processes, support personalised medicine approaches, and facilitate more detailed investigations into cellular behaviour within 3D structures.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Models; Tools
ISSN (print) / ISBN
2041-1723
e-ISSN
2041-1723
Journal
Nature Communications
Quellenangaben
Volume: 16,
Issue: 1,
Article Number: 8897
Publisher
Nature Publishing Group
Publishing Place
London
Reviewing status
Peer reviewed
Institute(s)
Human-Centered AI (HCA)
Grants
HUNRENTECH
Horizon-BIALYMPH
Horizon-SYMMETRY
Horizon-SWEEPICS
H2020-Fair-CHARM
HAS-NAP3
TKCS
PerMel-AI
HUNTER-Excellence 2024
OTKA-SNN
NKKP
FIMM High Content Imaging and Analysis Unit
Finnish Cancer Society
MAECI Science and Technology Cooperation Italy-South Korea by the Italian Ministry of Foreign Affairs and International Cooperation
Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences
Lenduelet BIOMAG
HUNRENTECH
Horizon-BIALYMPH
Horizon-SYMMETRY
Horizon-SWEEPICS
H2020-Fair-CHARM
HAS-NAP3
TKCS
PerMel-AI
HUNTER-Excellence 2024
OTKA-SNN
NKKP
FIMM High Content Imaging and Analysis Unit
Finnish Cancer Society
MAECI Science and Technology Cooperation Italy-South Korea by the Italian Ministry of Foreign Affairs and International Cooperation
Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences
Lenduelet BIOMAG