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Wasserer, S.* ; Litman, T.* ; Hebsgaard, J.* ; Jargosch, M. ; Hillig, C. ; Pilz, A.C.* ; Garzorz-Stark, N.* ; Biedermann, T.* ; Blanchetot, C.* ; Menden, M.P. ; Mortensen, M.S.* ; Skak-Nielsen, T.* ; Bertelsen, M.* ; Ursoe, B.* ; Lauffer, F.* ; Martel, B.C.* ; Eyerich, K.* ; Eyerich, S.

Neutralizing IL-22RA1 improves histological and molecular alterations associated with atopic dermatitis pathogenesis.

J. Allergy Clin. Immunol., DOI: 10.1016/j.jaci.2025.08.033 (2025)
Postprint DOI PMC
Open Access Green
BACKGROUND: A subgroup of patients with atopic dermatitis (AD) do not show sufficient improvement with current systemic therapies, highlighting the heterogeneity of the chronic inflammatory skin disease and the need for novel treatments. OBJECTIVES: In this study, we investigated the pathogenic contribution of the IL-22/IL-22 receptor (IL-22RA1) axis to AD skin inflammation in in vitro, ex vivo, and in vivo models to evaluate the therapeutic potential of blocking this axis. METHODS: IL-22RA1 expression in AD skin was assessed by in situ hybridization. Inhibition of the IL-22/IL-22R signaling cascade was evaluated in a human AD in vitro model (3D skin equivalents) and a TPA mouse model using temtokibart, a humanized antibody directed against IL-22RA1. RESULTS: IL22RA1 was highly expressed in the epidermis of lesional AD skin versus non-lesional control skin; expression correlated positively with epidermal thickness and negatively with the barrier integrity marker loricrin. IL-22 stimulation in 3D skin equivalents induced a specific molecular signature associated with lack of terminal differentiation, altered lipid metabolism, and increased immune response. Inhibition of IL-22RA1 with temtokibart showed significant improvements in skin barrier integrity at the histological and molecular levels. IL-22RA1 inhibition in a skin inflammation mouse model with Zymo, a surrogate murine anti-IL-22RA1 monoclonal antibody for temtokibart, reduced local expression of Cxcl1 and S100a9. CONCLUSIONS: These findings suggest that the IL-22/IL-22RA1 axis functionally contributes to AD pathogenesis. Thus, blocking IL-22RA1 represents a potentially valuable new therapeutic option.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Il-22 ; Il-22ra1 ; Atopic Dermatitis ; Skin Inflammation ; Temtokibart ; Treatment
ISSN (print) / ISBN 0091-6749
e-ISSN 1097-6825
Journal The journal of allergy and clinical immunology
Publisher Elsevier
Publishing Place Amsterdam [u.a.]
Reviewing status Peer reviewed
Institute(s) Institute for Allergy Research (IAF)
Institute of Computational Biology (ICB)