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Feiten, A.F.* ; Dahm, K.* ; Schlepckow, K.* ; van Lengerich, B.* ; Suh, J.H.* ; Reifschneider, A.* ; Wefers, B.* ; Bartos, L.M.* ; Wind-Mark, K.* ; de Weerd, L.* ; Ulas, T.* ; De-Domenico, E.* ; Grundschöttel, P.* ; Paulusch, S.* ; Tast, B.* ; Honda, T.* ; Müller, S.A.* ; Becker, M.* ; Khalin, I.* ; Ricci, A.* ; Liesz, A.* ; Brunner, B.* ; Krenner, C.* ; Buschmann, K.* ; Nuscher, B.* ; Spieth, L.* ; Junker, N.* ; Berghoff, S.A.* ; Davis, S.S.* ; Neher, J.J.* ; Wurst, W. ; Plesnila, N.* ; Lewcock, J.W.* ; Simons, M.* ; Lichtenthaler, S.F.* ; Di Paolo, G.* ; Brendel, M.* ; Capell, A.* ; Monroe, K.M.* ; Schultze, J.L.* ; Haass, C.*

TREM2 expression level is critical for microglial state, metabolic capacity and efficacy of TREM2 agonism.

Nat. Commun. 17:2002 (2026)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Triggering receptor expressed on myeloid cells 2 (TREM2) is a central regulator of microglial activity and loss-of-function coding variants are major risk factors for late onset Alzheimer's disease (LOAD). To better understand the molecular and functional changes associated with TREM2 signalling in microglia, we generated a TREM2 reporter mouse. In APP transgenic animals, bulk RNA-sequencing of isolated microglia sorted based on reporter expression highlighted TREM2 level-related changes in major immunometabolic pathways, and enrichment of genes in oxidative phosphorylation and cholesterol metabolism in microglia with increased TREM2 expression. Metabolic and lipidomic profiling of sorted microglia showed that, independent of Aβ pathology, TREM2 expression correlated with signatures consistent with increased cellular redox, energetics, and cholesterol homoeostasis. In accordance, metabolic activity correlated with phagocytic capacity. Finally, we performed chronic treatment with a TREM2 agonist antibody and identified a window of TREM2 expression where microglia are most responsive, thereby informing clinical applications of TREM2 agonists.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Trem2 ; Microglia ; Receptor ; Reporter Gene ; Regulator ; Agonist ; Transgene ; Gene ; Phosphorylation
ISSN (print) / ISBN 2041-1723
e-ISSN 2041-1723
Journal Nature Communications
Quellenangaben Volume: 17, Issue: 1, Pages: , Article Number: 2002 Supplement: ,
Publisher Springer
Publishing Place London
Reviewing status Peer reviewed
Institute(s) Institute of Stem Cell Research (ISF)