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Protocol for measuring mitochondrial respiration in mature human adipocytes using the Seahorse XF analyzer.
STAR Protoc. 7:104520 (2026)
Primary mature human adipocytes are an important tool bridging basic research and clinical medicine by reflecting human biological diversity. Here, we present a protocol for measuring the oxygen consumption rate of mature adipocytes. We describe the steps for isolation of adipocytes from adipose tissue samples, Matrigel embedding, and Seahorse analysis, as well as normalization and data analysis. This protocol enables the investigation of the metabolic function of primary mature human adipocytes in response to drugs or genetic modifications.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Cell Biology ; Cell Culture ; Cell Isolation ; Metabolism
e-ISSN
2666-1667
Journal
STAR Protocols
Quellenangaben
Volume: 7,
Issue: 2,
Article Number: 104520
Publisher
Elsevier
Publishing Place
50 Hampshire St, Floor 5, Cambridge, Ma 02139 Usa
Reviewing status
Peer reviewed
Institute(s)
Helmholtz Institute for Metabolism, Obesity and Vascular Research (HI-MAG)
Grants
Deutsche Forschungsgemeinschaft
Studienstiftung des Deutschen Volkes
Studienstiftung des Deutschen Volkes