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Armbrust, N. ; Grosshauser, M. ; Geilenkeuser, J. ; Stroppel, L.* ; Jozinovic, M.* ; Levermann, H.* ; Panne, T.* ; Wißmann, J.* ; Goelitz, L.* ; Schmidt, S. ; Orschmann, T. ; Rusha, E. ; Steinmaßl, E.* ; Widenmeyer, F.* ; Warsing, N.* ; Sabry, M.* ; Sultanbai, A.* ; Santl, T. ; Geerlof, A. ; Berezin, O.* ; Bodea, S.V. ; Moretti, A.* ; Schneider, S. ; Theis, F.J. ; Gagneur, J.* ; Truong, D.J.J. ; Westmeyer, G.G.

Non-destructive transcriptomics via vesicular export.

Nat. Commun. 17:3812 (2026)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Transcriptomics enables comprehensive, multiplexed characterization of cellular states, yet prevailing methods typically require cell fixation or lysis, precluding longitudinal analysis of RNA expression in living cells. Here, we present non-destructive transcriptomics by vesicular export (NTVE), a platform for multi-time-point monitoring of RNA expression dynamics in living cells. Stabilized RNA reporter barcodes can be selectively packaged and exported from cells via virus-like particles (VLPs) bearing bioorthogonal affinity handles for convenient multichannel tracking of co-cultured cells. Using an engineered poly(A)-binding protein adapter, NTVE exports endogenous transcripts from inducible human and murine cell lines with high concordance to conventional lysate-derived RNA-seq. NTVE captures transcriptome changes in response to genetic and chemical perturbations within the same cells over time using standard sequencing workflows. NTVE can further be equipped with fusogens to deliver mRNA-encoded effectors or ribonucleoprotein gene editors from sender cells, activating gene reporters in co-cultured recipient cells. We demonstrate the utility of NTVE for monitoring hiPSC differentiation through daily non-destructive transcriptomic profiling of lineage-specific marker dynamics.
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Publication type Article: Journal article
Document type Scientific Article
ISSN (print) / ISBN 2041-1723
e-ISSN 2041-1723
Journal Nature Communications
Quellenangaben Volume: 17, Issue: , Pages: , Article Number: 3812 Supplement: ,
Publisher Springer
Publishing Place London
Reviewing status Peer reviewed
Institute(s) Insitute of Synthetic Biomedicine (ISBM)
Institute of Structural Biology (STB)
Institute of Computational Biology (ICB)