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Global dataset of soil eukaryotic communities created with a uniform protocol and long read sequencing.
Sci. Data, DOI: 10.1038/s41597-026-07315-y (2026)
Soil eukaryotes, including fungi, protists, plants, and animals, are central to biosphere functioning and resilience. The Global Standardised Soil Eukaryome Dataset (GloSED) is the first dataset encompassing the entire spectrum of soil eukaryotes, covering 4,063 sampling sites in 121 countries on all continents, revealing nearly one million operational taxonomic units. All samples were collected and analysed using a standardised protocol minimizing technical biases. Long-read sequencing of full-length ITS and 18S-V9 regions provide broad taxonomic coverage and high-resolution identification supported by specialist curation of "dark taxa". A rigorous bioinformatic processing ensures against homopolymer errors, PCR-mediated chimeras, and index switching providing high data quality. The dataset is supported by raw sequences and an open-source containerised workflow for reproducible analyses. The samples are accompanied by land-cover description and directly measured soil pH, δ13C, δ15N, as well as P, K, Ca, Mg, and total C and N contents. GloSED is the first database that enables ecological and biogeographic studies of entire soil eukaryotic communities from local to global scales.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
2052-4463
e-ISSN
2052-4463
Journal
Scientific Data
Publisher
Springer
Publishing Place
London
Reviewing status
Peer reviewed
Institute(s)
Research Unit Environmental Simulation (EUS)