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Nucleotide-dependent switching and RIPb effector recognition of the barley susceptibility factor RACB.
Comm. Biol. 9:691 (2026)
ROP proteins are plant-specific members of the Rho family of small GTPases that orchestrate fundamental signaling pathways controlling cell polarity, directional growth, and immune responses. Although their biological importance is well established, the structural basis underlying their activation and interactions with downstream effectors has remained insufficiently understood. Here, we present an atomic-resolution structural analysis of RACB, a ROP GTPase from barley (Hordeum vulgare) that functions as a key susceptibility factor during fungal infection. Using an integrative approach combining X-ray crystallography, nuclear magnetic resonance spectroscopy, and hydrogen-deuterium exchange mass spectrometry, we capture high-resolution structural and dynamical snapshots of RACB in both its inactive and active conformations. This setup reveals the conformational flexibility and switching mechanism that are central to RACB function. Moreover, the structure of the complex between active RACB and its effector protein RIPb uncovers the fully activated state of RACB and identifies a conserved interaction motif within RIPb that mediates complex formation, providing mechanistic insights into how RIPb can link membrane-associated RACB to the microtubule cytoskeleton to facilitate membrane remodeling processes. These findings establish a detailed structural framework for plant Rho-type GTPase signaling and offer a molecular explanation for how pathogens exploit ROP-mediated pathways to promote infection in plants.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Effector ; Gtpase ; Signal Transduction ; Cytoskeleton ; Gtpase-activating Protein ; Protein–protein Interaction ; Microtubule ; Cdc42 ; Structural Biology; Powdery Mildew Fungus; Exchange Mass-spectrometry; Functional-analysis; Crystal-structure; Epidermal-cells; Gtpase; Protein; Rop; Rho; Dynamics
ISSN (print) / ISBN
2399-3642
e-ISSN
2399-3642
Journal
Communications Biology
Quellenangaben
Volume: 9,
Issue: 1,
Article Number: 691
Publisher
Springer
Publishing Place
London
Reviewing status
Peer reviewed
Institute(s)
Institute of Structural Biology (STB)
Grants
Deutsche Forschungsgemeinschaft (German Research Foundation)