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Schmitt, S.* ; Komlódi, T.* ; Rychtárová, L.* ; Donnelly, C.* ; Zischka, H. ; Gnaiger, E.*

Oxygen dependence of hydrogen peroxide production in isolated mitochondria and permeabilized cells.

In: (SFRR-E Annual Meeting 2026 “Redox Biology, Environmental Exposure and Lifestyle”). New York, NY: Elsevier, 2026. S47 - S47 (Free Radical Biol. Med. ; 249)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
(Poly)phenols are natural antioxidants found in foods, which have been associated with health benefits. Their ability to inhibit the formation of advanced glycation end products (AGEs) has been attributed to multiple mechanisms, including scavenging of reactive oxygen species and dicarbonyl compounds. However, limited information is available regarding how thermal treatment (TT) under different oxidation conditions affects (poly)phenol composition and, consequently, their antiglycation activity and protein protection mechanisms. In this work, changes in chemical composition and antiglycation mechanisms of (poly)phenol-enriched extracts (PEE) from Chilean blackcurrant (Ribes cucullatum) were evaluated after exposure to different temperatures (4°C and 90°C) at two oxygen condition (0% and 20% O2) in buffer pH 7.4. (Poly)phenolic composition and redox properties were characterized by HPLC-DAD-MS and cyclic voltammetry, respectively. Antiglycation activity was assessed by incubating bovine serum albumin (BSA) with glucose and different concentrations of PEE and determining IC50 values of fluorescence inhibition (ex=325/em=440). Samples exposed to 20% O2 were more oxidized than those exposed to 0% O2, and this promoted the isomerization and glycosylation of phenolic compounds when the PEE was thermally treated. These changes enhanced antioxidant capacity (DPPH and ORAC assays), increased methylglyoxal-trapping ability, and decreased the levels of carbonyl protein formation in thermally treated PEE. In contrast, PEE thermally treated at 90°C under 0% O2 showed lower IC50 values for fluorescent AGEs compared with samples treated at 20% O2. HPLC-DAD analysis after incubation with BSA with PEE for 40h, showed that PEE incubated at 0% O2 possesses a higher association with BSA when compared with PEE incubated at 20% O2, indicating different interaction association of (poly)phenols to BSA, which could likely affect the protein glycation. Overall, these results indicate that TT of (poly)phenols from Ribes cucullatum increases the antioxidant and antiglycation activities, compared without TT, highlighting the relevance of oxidation during thermal treatment in modulating antiglycation efficiency.Supported by: This work received funding from ANID for a doctoral grant Folio 21230336, FONDECYT 1221280 (F. Ávila), and the German Research Foundation (DFG), Project number JI 411/1-1. (F. Jiménez-Aspee)
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Publication type Article: Conference contribution
Keywords Hydrogen Peroxide ; Mitochondrion ; Oxygen ; Reactive Oxygen Species ; Peroxide
ISSN (print) / ISBN 0891-5849
e-ISSN 1873-4596
Conference Title SFRR-E Annual Meeting 2026 “Redox Biology, Environmental Exposure and Lifestyle”
Journal Free Radical Biology and Medicine
Quellenangaben Volume: 249, Issue: , Pages: S47 - S47 Article Number: , Supplement: ,
Publisher Elsevier
Publishing Place New York, NY
Reviewing status Peer reviewed
Institute(s) Institute of Molecular Toxicology and Pharmacology (TOX)