Home
Login
Deutsch
Advanced Search
Browse by ...
Publication overview
Contact persons
Help
Publ. Version/Full Text
Research data
DOI
 |
Open Access Hybrid |
|
as soon as is submitted to ZB.
Fibrin hydrogels regulate human astrocyte state and neuronal reprogramming.
Cell Biomater., DOI: 10.1016/j.celbio.2026.100493 (2026)
Astrocytes are key components in reactive gliosis after brain injury, yet defined in vitro models dissecting the influence of extracellular matrix components enriched after injury, such as fibrin, on human astrocyte behavior and function are still missing. Here, we use fibrinogen-derived fibrin and fibrin-alginate-RGD (FAR) 3D hydrogel substrates to examine their influence on human induced pluripotent stem cell (hiPSC)-derived astrocyte behavior and on their direct conversion into neurons. Astrocytes develop complex morphologies in 3D-FAR hydrogels, while they are more proliferative and migratory in 3D-fibrin hydrogels (3D-fibrin). Interestingly, gene expression analysis revealed different reactive states of astrocytes in 3D-fibrin and 3D-FAR, which persist over time. The highly inflammatory state and stiffness of 3D-FAR are largely incompatible with direct neuronal reprogramming, hampering the direct conversion even at early stages. Conversely, astrocytes in 3D-fibrin hydrogels can convert into neuronal cells, demonstrating a potent influence of how fibrin is presented on distinct astrocyte states, with great relevance for fate conversion.
Altmetric
Additional Metrics?
Edit extra informations
Login
Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Astrocytes ; Biomaterials ; Fibrin ; Glia ; Human ; Hydrogels ; Ipsc ; Reprogramming ; Rna Sequencing
ISSN (print) / ISBN
3050-5623
e-ISSN
3050-5623
Journal
Cell Biomaterials
Publisher
Elsevier
Reviewing status
Peer reviewed
Institute(s)
Institute of Stem Cell Research (ISF)