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Ansel, K.M.* ; Pastor, W.A.* ; Rath, N. ; Lapan, A.D.* ; Glasmacher, E. ; Wolf, C. ; Smith, L.C.* ; Papadopoulou, N. ; Lamperti, E.D.* ; Tahiliani, M.* ; Ellwart, J.W. ; Shi, Y.* ; Kremmer, E. ; Rao, A.* ; Heissmeyer, V.

Mouse Eri1 interacts with the ribosome and catalyzes 5.8S rRNA processing.

Nat. Struct. Mol. Biol. 15, 523-530 (2008)
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Eri1 is a 3'-to-5' exoribonuclease conserved from fission yeast to humans. Here we show that Eri1 associates with ribosomes and ribosomal RNA (rRNA). Ribosomes from Eri1-deficient mice contain 5.8S rRNA that is aberrantly extended at its 3' end, and Eri1, but not a catalytically inactive mutant, converts this abnormal 5.8S rRNA to the wild-type form in vitro and in cells. In human and murine cells, Eri1 localizes to the cytoplasm and nucleus, with enrichment in the nucleolus, the site of preribosome biogenesis. RNA binding residues in the Eri1 SAP and linker domains promote stable association with rRNA and thereby facilitate 5.8S rRNA 3' end processing. Taken together, our findings indicate that Eri1 catalyzes the final trimming step in 5.8S rRNA processing, functionally and spatially connecting this regulator of RNAi with the basal translation machinery.
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Publication type Article: Journal article
Document type Scientific Article
Language english
Publication Year 2008
HGF-reported in Year 2008
ISSN (print) / ISBN 1545-9993
e-ISSN 1545-9985
Journal Nature Structural & Molecular Biology
Quellenangaben Volume: 15, Issue: 5, Pages: 523-530 Article Number: , Supplement: ,
Publisher Nature Publishing Group
Publishing Place New York, NY
Reviewing status Peer reviewed
Institute(s) Institute of Molecular Immunology (IMI)
Institute of Diabetes and Obesity (IDO)
Research Unit Molecular Immune Regulation (AMIR)
POF-Topic(s)
30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
30502 - Diabetes: Pathophysiology, Prevention and Therapy
Research field(s)
Immune Response and Infection
Helmholtz Diabetes Center
PSP Element(s) G-551000-001
G-501792-001
G-501793-001
G-553100-001
DOI 10.1038/nsmb.1417
WOS ID 000255587800021
Scopus ID 43249083987
PubMed ID 18438418
Erfassungsdatum 2008-08-27
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