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Synthetic rhizosphere bacterial communities induce systemic resistance to barley powdery mildew without major shifts in the native bacterial community.
Front. Microbiol. 17:1818676 (2026)
Introduction: Synthetic
microbial communities (SynComs) could help plants withstand biotic
stress and reduce the need for pesticides. However, it remains unclear
whether SynComs composed of host- or non-host-associated rhizosphere
bacteria can trigger induced systemic resistance (ISR) in barley without
causing major shifts in the native rhizosphere bacterial community.Methods: Here,
we constructed two SynComs with known strain composition, composed of
bacterial strains isolated from the host-associated barley rhizosphere
and non-host-associated wheat rhizosphere. Their ability to trigger
induced systemic resistance (ISR) against the barley powdery mildew
pathogen Blumeria graminis f. sp. hordei (Bgh) was tested. To
investigate plant-microbe interactions from both plant and microbial
perspectives, we quantified Bgh propagation in leaves by DAF staining,
analysed leaf transcriptomes, and profiled the rhizosphere microbiome
using 16S rRNA gene amplicon sequencing and metatranscriptomics.Results: Both
SynComs reduced fungal growth in barley leaves to a similar extent as
the positive control strain, Pseudomonas simiae WCS417r, suggesting that
ISR-like protection can also be achieved by defined multi-strain
communities. Although both SynComs provided similar overall protection,
the barley SynCom exhibited the strongest numerical reduction in fungal
growth. These findings build on previous single-strain ISR studies and
suggest that community-mediated protection is not restricted to
host-derived bacterial consortia. Inoculations with both SynComs and
WCS417r were not associated with statistically significant changes in
the rhizosphere bacterial community structure. All treatments induced
only subtle pre-infection transcriptional responses in barley leaves
that were consistent with ISR-mediated priming. However, treatment with
WCS417r yielded a higher number of differentially expressed genes than
either SynCom. Rhizosphere metatranscriptomics revealed
treatment-specific functional shifts. The two features K05516 and
PF02868 were affected by all three treatments, implying the existence of
shared changes related to stress adaptation and microbial activity.
OTUs matching the inoculated SynCom members were still present in the
rhizosphere at harvest, suggesting the persistence of at least some of
the introduced communities.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Rhizosphere ; Blumeria Graminis ; Powdery Mildew ; Microbiome ; Hordeum Vulgare ; Pseudomonas Syringae ; Pseudomonas ; Bacteria ; Microbial Population Biology; Plant-growth; Ribosomal-rna; Responses; Soil; Immunity; Defense; Photosynthesis; Transcriptome; Rhizobacteria; Pathogens
ISSN (print) / ISBN
1664-302X
e-ISSN
1664-302X
Journal
Frontiers in Microbiology
Quellenangaben
Volume: 17,
Article Number: 1818676
Publisher
Frontiers
Publishing Place
Avenue Du Tribunal Federal 34, Lausanne, Ch-1015, Switzerland
Reviewing status
Peer reviewed
Institute(s)
Institute of Biochemical Plant Pathology (BIOP)
Grants
German Research Foundation (DFG)