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Guedes, J.* ; Woldmar, N.* ; Baltás, E.* ; Kriston, A.* ; Migh, E.* ; Kovács, F.* ; Oskolas, H.* ; Marko-Varga, M.* ; Appelqvist, R.* ; Wieslander, E.* ; Szasz, A.M.* ; Veréb, Z.* ; Gyulai, R.* ; Németh, I.B.* ; Malm, J.* ; Horvath, P. ; Gil, J.* ; Marko-Varga, G.*

AI-based digital pathology and spatial proteomics enable precision oncology: A case report of recurrent melanoma in a young patient.

npj Precis. Oncol. 10, 14:296 (2026)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Metastatic melanoma presents clinical challenges due to tumor heterogeneity and treatment resistance. Here, we report an integrative workflow combining AI-based digital pathology with spatial proteomics to support personalized treatment strategies in a case of a young patient with recurrent melanoma and multiple metastases. Our AI model trained on H&E images identified two spatially separated cell subpopulations (PT1 and PT2) within the primary lesion, along with metastatic areas and stromal components. MS-based proteomics was used to map the spatial proteome across the clinically relevant regions. Our findings indicate inter-tumor heterogeneity and increased kinases associated with target-drug resistance. Convergent morphological and proteomic signatures identified PT1 as an aggressive melanoma subtype and the likely metastatic driver. Augmented glycolytic signaling and mitochondrial metabolism were identified as drivers of melanoma progression in this patient. Our findings suggest that targeted therapies may provide limited benefit, while the combination with metabolic inhibitors could represent a more effective treatment option for the patient.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Proteomics ; Melanoma ; Metastatic Melanoma ; Proteome ; Stromal Cell ; Digital Pathology ; Workflow ; Precision Medicine
ISSN (print) / ISBN 2397-768X
e-ISSN 2397-768X
Journal npj Precision Oncology
Quellenangaben Volume: 10, Issue: 1, Pages: 14, Article Number: 296 Supplement: ,
Publisher Springer
Publishing Place Heidelberger Platz 3, Berlin, 14197, Germany
Reviewing status Peer reviewed
Institute(s) Institute of AI for Health (AIH)
Grants Berta Kamprad Foundation and the Crafoord Foundation
Nemzeti Kutatási Fejlesztési és Innovációs Hivatal
Apotekarsocieteten