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Publ. Version/Full Text
Publ. Version/Full Text
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Optical and photochemical properties of suwannee river natural organic matter fractions generated by preparative free flow electrophoresis.
Environ. Sci. Technol., DOI: 10.1021/acs.est.6c07200 (2026)
Publ. Version/Full Text
Publ. Version/Full Text
DOI
Fractionation of
dissolved organic matter (DOM) has been used for
decades to simplify the challenging task of bulk sample characterization
and to gain insight into the structural features governing DOM reactivity.
Here, we used preparative free-flow electrophoresis (PFFE), which
separates molecules based on their charge-to-size ratio, to separate
Suwannee River natural organic matter (SRNOM) into fractions with
varying electrophoretic mobility. Optical and photochemical data of
the fractions were paired with extensive analytical characterization.
The least electrophoretically mobile fraction had the lowest number-average
molecular weight (Mn), aromaticity, and
specific ultraviolet absorbance (SUVA254), while showing
the largest quantum yields of fluorescence as well as singlet oxygen
and hydroxyl radical formation. Structure–activity relationships
for the fractions, which exhibited substantial differences in mobility,
aromaticity, and Mn did not follow the
conventional trends observed in size-based DOM fractionation studies
(e.g., SUVA254 ∝ aromaticity). These findings highlight
the role of charge and structural differences in driving optical and
photochemical properties apart from bulk aromaticity and Mn. The distribution of absorbance, the sum of vanillyl
and syringyl phenols, and DIOHBA (3,5-dihydroxybenzoic acid) indicates
that the most electrophoretically mobile fractions with the highest
absorbance are dominated by DIOHBA, a phenol monomer derived from
tannins and flavonoids, rather than lignin. DIOHBA was inversely related
to the fluorescence index and β/α. Overall, these results
provide insight into SRNOM photophysics and highlight the utility
of PFFE as a tool for resolving DOM structure–reactivity relationships.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Fractionation ; Absorbance ; Singlet Oxygen ; Fluorescence ; Dissolved Organic Carbon ; Electrophoresis ; Fraction (chemistry) ; Phenol
ISSN (print) / ISBN
0013-936X
e-ISSN
1520-5851
Publisher
American Chemical Society (ACS)
Publishing Place
Washington, DC
Reviewing status
Peer reviewed
Institute(s)
Research Unit BioGeoChemistry and Analytics (BGC)