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Höfig, I. ; Ehrhardt, H. ; Jeremias, I.

Efficient RNA interference in patients' acute lymphoblastic leukemia cells amplified as xenografts in mice.

Cell Commun. Signal. 10:8 (2012)

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Background: Signaling studies in cell lines are hampered by non-physiological alterations obtained in vitro. Physiologic primary tumor cells from patients with leukemia require passaging through immune-compromised mice for amplification. The aim was to enable molecular work in patients' ALL cells by establishing siRNA transfection into cells amplified in mice. Results: We established delivering siRNA into these cells without affecting cell viability. Knockdown of single or multiple genes reduced constitutive or induced protein expression accompanied by marked signaling alterations. Conclusion: Our novel technique allows using patient-derived tumor cells instead of cell lines for signaling studies in leukemia.

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0.746