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Open Access Green as soon as Postprint is submitted to ZB.
Comprehensive proteome analysis in Cenococcum geophilum Fr. as a tool to discover drought-related proteins.
J. Proteomics 75, 3707-3719 (2012)
Cenococcum geophilum is a widely distributed ectomycorrhizal fungus potentially playing a significant role in resistance and resilience mechanisms of its tree hosts exposed to drought stress. In this study, we performed a large scale protein analysis in pure cultures of C. geophilum in order to gain first global insights into the proteome assembly of this fungus. Using 1-D gel electrophoresis coupled with ESI-MS/MS, we indentified 638 unique proteins. Most of these proteins were related to the metabolic and cellular processes, and the transport machinery of cells. In a second step, we examined the influence of water deprivation on the proteome of C. geophilum pure cultures at three time points of gradually imposed drought. The results indicated that 12 proteins were differentially abundant in mycelia subjected to drought compared to controls. The induced responses in C. geophilum point towards regulation of osmotic stress, maintainance of cell integrity, and counteracting increased levels of reactive oxygen species formed during water deprivation.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Cenococcum Geophilum ; Drought ; Abiotic Stress ; Fungal Proteomics; POLYPEPTIDE-ASSOCIATED COMPLEX; STRESS-RESPONSIVE PROTEINS; MYCORRHIZAL FUNGI; SACCHAROMYCES-CEREVISIAE; TREHALOSE BIOSYNTHESIS; ARABIDOPSIS-THALIANA; SHOTGUN PROTEOMICS; REPRODUCTIVE STAGE; ABIOTIC STRESS; WATER-DEFICIT
ISSN (print) / ISBN
1874-3919
e-ISSN
1876-7737
Journal
Journal of Proteomics
Quellenangaben
Volume: 75,
Issue: 12,
Pages: 3707-3719
Publisher
Elsevier
Non-patent literature
Publications
Reviewing status
Peer reviewed