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Open Access Gold as soon as Publ. Version/Full Text is submitted to ZB.
NC2 mobilizes TBP on core promoter TATA boxes.
Nat. Struct. Mol. Biol. 14, 1196-1201 (2007)
The general transcription factors (GTFs) of eukaryotic RNA polymerase II, in a process facilitated by regulatory and accessory factors, target promoters through synergistic interactions with core elements. The specific binding of the TATA box-binding protein (TBP) to the TATA box has led to the assumption that GTFs recognize promoters directly, producing a preinitiation complex at a defined position. Using biochemical analysis as well as biophysical single-pair Förster resonance energy transfer, we now provide evidence that negative cofactor-2 (NC2) induces dynamic conformational changes in the TBP-DNA complex that allow it to escape and return to TATA-binding mode. This can lead to movement of TBP along the DNA away from TATA.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
DNA/chemistry; Electrophoretic Mobility Shift Assay; Fluorescence Resonance Energy Tansfer;Humans; Molecular Structure; Phosphoproteins/chemistry; Phosphoproteins/physiology*; Promoter Regions; Genetic*; TATA Box* TATA-Box Binding; Protein/chemistry
ISSN (print) / ISBN
1545-9993
e-ISSN
1545-9985
Quellenangaben
Volume: 14,
Issue: 12,
Pages: 1196-1201
Publisher
Nature Publishing Group
Publishing Place
New York, NY
Reviewing status
Peer reviewed
Institute(s)
Institute of Molecular Immunology (IMI)