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Open Access Green as soon as Postprint is submitted to ZB.
Development of a primer system to study abundance and diversity of the gene coding for alanine aminopeptidase pepN gene in gram-negative soil bacteria.
J. Microbiol. Methods 91, 14-21 (2012)
A new set of primers was developed allowing the specific detection of the pepN gene (coding for alanine aminopeptidase) from Gram-negative bacteria. The primers were designed in silico by sequence alignments based on available DNA sequence data. The PCR assay was validated using DNA from selected pure cultures. The analysis of gene libraries from extracted DNA from different soil samples revealed a high diversity of pepN related sequences mainly related to α-Proteobacteria. Most sequences obtained from clone libraries were closely related to already published sequences (<80% homology on amino acid level), which may be related to the conserved character of the amplified region of pepN. By linking the diversity data obtained by the clone library studies to potential enzymatic activities of alanine aminopeptidase, lowest diversity of pepN was found in those soil samples which displayed lowest activity levels, which confirms the importance of diversity for the ecosystem function mainly when transformation processes of complex molecules are studied.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Alanine aminopeptidase; pepN gene; Environmental samples; PCR primer; Proteobacteria
ISSN (print) / ISBN
0167-7012
e-ISSN
1872-8359
Quellenangaben
Volume: 91,
Issue: 1,
Pages: 14-21
Publisher
Elsevier
Publishing Place
Amsterdam
Reviewing status
Peer reviewed
Institute(s)
Comparative Microbiome Analysis (COMI)