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Sharma, S.* ; Mehta, R.* ; Gupta, R.* ; Schloter, M.

Improved protocol for the extraction of bacterial mRNA from soils.

J. Microbiol. Methods 91, 62-64 (2012)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
An improved protocol for extraction of prokaryotic mRNA from soil samples was developed by modifying the extraction procedure to obtain higher yields of mRNA and to reduce co-extraction of humic acids. The modified protocol was found to be more robust and efficient compared to the original protocol by Griffiths et al. (2000) without compromising with the quality and quantity of RNA.
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Publication type Article: Journal article
Document type Scientific Article
Keywords mRNA; Soil; glnA
ISSN (print) / ISBN 0167-7012
e-ISSN 1872-8359
Quellenangaben Volume: 91, Issue: 1, Pages: 62-64 Article Number: , Supplement: ,
Publisher Elsevier
Publishing Place Amsterdam
Reviewing status Peer reviewed