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Improved protocol for the extraction of bacterial mRNA from soils.
J. Microbiol. Methods 91, 62-64 (2012)
An improved protocol for extraction of prokaryotic mRNA from soil samples was developed by modifying the extraction procedure to obtain higher yields of mRNA and to reduce co-extraction of humic acids. The modified protocol was found to be more robust and efficient compared to the original protocol by Griffiths et al. (2000) without compromising with the quality and quantity of RNA.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
mRNA; Soil; glnA
ISSN (print) / ISBN
0167-7012
e-ISSN
1872-8359
Quellenangaben
Volume: 91,
Issue: 1,
Pages: 62-64
Publisher
Elsevier
Publishing Place
Amsterdam
Reviewing status
Peer reviewed
Institute(s)
Comparative Microbiome Analysis (COMI)