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Kneilling, M.* ; Mailhammer, R. ; Hültner, L. ; Schönberger, T.* ; Fuchs, K.* ; Schaller, M.* ; Bukala, D.* ; Massberg, S.* ; Sander, C.A.* ; Braumüller, H.* ; Eichner, M.* ; Maier, K.L. ; Hallmann, R.* ; Pichler, B.J.* ; Haubner, R.* ; Gawaz, M. ; Pfeffer, K.* ; Biedermann, T.* ; Röcken, M.*

Direct crosstalk between mast cell-TNF and TNFR1-expressing endothelia mediates local tissue inflammation.

Blood 114, 1696-1706 (2009)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Signaling through tumor necrosis factor receptor 1 (TNFR1) controls bacterial infections and the induction of inflammatory Th1 cell-mediated autoimmune diseases. By dissecting Th1 cell-mediated delayed-type hypersensitivity responses (DTHRs) into single steps, we localized a central defect to the missing TNFR1 expression by endothelial cells (Ecs). Adoptive transfer and mast cell knockin experiments into Kit(W)/Kit(W-v), TNF-/-, and TNFR1(-/-) mice showed that the signaling defect exclusively affects mast cell-EC interactions but not T cells or antigen-presenting cells. As a consequence, TNFR1(-/-) mice had strongly reduced mRNA and protein expression of P-selectin, E-selectin, ICAM-1, and VCAM-1 during DTHR elicitation. In consequence, intravital fluorescence microscopy revealed up to 80% reduction of leukocyte rolling and firm adhesion in TNFR1(-/-) mice. As substitution of TNF-/- mice with TNF-producing mast cells fully restored DTHR in these mice, signaling of mast cell-derived TNF through TNFR1-expressing Ecs is essential for the recruitment of leukocytes into sites of inflammation.
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Publication type Article: Journal article
Document type Scientific Article
Keywords tumor-necrosis-factor; contact hypersensitivity response; intercellular-adhesion molecule-1; versus-host-disease; factor-receptor; t-cells; p-selectin; mice deficient; ultraviolet-b; factor-alpha
Language english
Publication Year 2009
HGF-reported in Year 0
ISSN (print) / ISBN 0006-4971
e-ISSN 1528-0020
Journal Blood
Quellenangaben Volume: 114, Issue: 8, Pages: 1696-1706 Article Number: , Supplement: ,
Publisher American Society of Hematology
Publishing Place Washington
Reviewing status Peer reviewed
Institute(s) Institute of Clinical Molecular Biology and Tumor Genetics (K.MOLBI)
Institute of Lung Health and Immunity (LHI)
POF-Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
30202 - Environmental Health
Research field(s) Immune Response and Infection
Lung Research
PSP Element(s) G-501400-005
G-505000-001
PubMed ID 19546478
DOI 10.1182/blood-2008-11-187682
Scopus ID 70349566472
Erfassungsdatum 2009-12-31
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