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Caldwell, R.B. ; Fiedler, P. ; Schoetz, U. ; Buerstedde, J.-M.

Gene Function Analysis Using the Chicken B-Cell Line DT40.

In: Gene Function Analysis. Berlin [u.a.]: Springer, 2007. 193-210 (Methods Mol. Biol. ; 408)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
Quidquid agis, prudenter agas et respice finem!—Whatever you do, do it wisely and consider the goal. In consideration of that sage advice, the chicken B-cell line DT40 is an excellent model cell system to study the function of vertebrate genes. In addition to being highly amenable to gene manipulations, the recent influx of genome and gene/protein resources allows for the straightforward selection, design, and targeting of candidate genes for knockout analysis. This chapter will give a step by step standardized protocol to creating a gene knockout mutant in DT40. With careful consideration, the methods and protocols described herein can be easily modified to allow for further gene manipulations such as creating a knockin or a conditional mutant.
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Publication type Article: Edited volume or book chapter
Editors Ochs, M.*
Keywords Conditional mutant; DT40; gene function analysis; gene manipulation; genotype; homologous recombination; knockin; knockout; phenotype; targeting
ISSN (print) / ISBN 1064-3745
e-ISSN 1940-6029
ISBN 978-1-58829-734-1
Book Volume Title Gene Function Analysis
Journal Methods in Molecular Biology
Quellenangaben Volume: 408, Issue: , Pages: 193-210 Article Number: , Supplement: ,
Publisher Springer
Publishing Place Berlin [u.a.]
Reviewing status Peer reviewed
Institute(s) Institute of Molecular Radiation Biology (IMS)