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Degenerate Oligonucleotide-Primed PCR.
Methods Mol. Biol. 226, 315-318 (2003)
The amount of genomic DNA available for genetic studies can often be limiting. Degenerated oligonucleotide-primed polymerase chain reaction (DOP-PCR) is an appropriate method for overcoming these limitations by efficiently performing whole genome amplification. The DOP-PCR technique is increasingly being applied for simultaneous amplification of multiple loci in target DNA using oligonucleotide primers of partially degenerate sequences. Contrary to other PCR-based general amplification methods (Alu-PCR or IRS-PCR), DOP-PCR is a species-independent technique for the amplification of small amounts of DNA.
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0.200
0.000
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Publication type
Article: Journal article
Document type
Scientific Article
Editors
Bartlett, J.M.S.* ; Stirling, D.*
Language
english
Publication Year
2003
HGF-reported in Year
0
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Conference Title
Methods in Molecular Biology : PCR Protocols
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 226,
Pages: 315-318
Publisher
Springer
Publishing Place
Berlin [u.a.]
Reviewing status
Peer reviewed
Institute(s)
Institute of Pathology (PATH)
Research field(s)
Enabling and Novel Technologies
PSP Element(s)
FE 70332
Erfassungsdatum
2003-09-10