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Babaryka, A. ; Kühn, E. ; Köster, R.W.

In vivo synthesis of meganuclease for generating transgenic zebrafish Danio rerio.

J. Fish Biol. 74, 452-457 (2009)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
The authors show that co-injection at the one-cell stage of mRNA encoding a nuclear-targeted meganuclease I-SceI together with expression cassettes flanked by cognate restriction sites results in efficient stable transgenesis in zebrafish Danio rerio.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Gal4; I-SceI; meganuclease; transgenesis; zebrafish; scei meganuclease; embryos
ISSN (print) / ISBN 0022-1112
e-ISSN 1095-8649
Journal Journal of Fish Biology
Quellenangaben Volume: 74, Issue: 2, Pages: 452-457 Article Number: , Supplement: ,
Publisher Wiley
Reviewing status Peer reviewed
Institute(s) Institute of Developmental Genetics (IDG)