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Open Access Green as soon as Postprint is submitted to ZB.
Practical considerations for the analysis of ionic and neutral organic molecules with capillary electrophoresis/mass spectrometry.
Methods Mol. Biol. 384, 135-156 (2008)
This chapter presents the technique of capillary electrophoresis coupled to mass spectrometry (CE/MS). The introductory section is targeted mainly at CE/MS beginners and notes briefly the theoretical background of electrospray ionization (ESI), the most commonly used ionization mode in CE/MS. The specifics of CE/MS are described—also in comparison with more classic methods like LC/MS. Important caveats to be taken nto consideration for successful CE/MS operation are noted in the interest of avoiding pitfalls. CE/MS is illustrated with three representative examples, which might serve as starting points for more in-detail experiments: (1) partial-filling micellar elektrokinetic chromatography (MEKC) ofneutral bacterial signaling molecules (N-acylhomoserine lactones) extracted from culture supernatants, (2) capillary zone electrophoresis (CZE) of their anionic degradation products, and finally (3) CZE separation of cationic hydroxys-triazines.
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Publication type
Article: Journal article
Document type
Scientific Article
Editors
Schmitt-Kopplin, P.
Keywords
capillary electrophoresis; mass spectrometry; electrospray ionization; coupling techniques; anions; cations; neutrals; N-acylhomoserine lactones; triazines
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Conference Title
Capillary Electrophoresis: Methods and Protocols
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 384,
Pages: 135-156
Series
Methods in Molecular Biology
Publisher
Springer
Publishing Place
Berlin [u.a.]
Reviewing status
Peer reviewed
Institute(s)
Institute of Ecological Chemistry (IOEC)