PuSH - Publication Server of Helmholtz Zentrum München

Ping, G.* ; Schmitt-Kopplin, P. ; Zhang, Y.* ; Baba, Y.*

Capillary electrochromatography and on-line concentration.

Methods Mol. Biol. 384, 751-769 (2008)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Capillary electrochromatography (CEC) is a micro-separation technique that combines the advantages of capillary zone electrophoresis with those of high-performance liquid chromatography. Accordingly, it has attracted extensive attention over the last decade. Among the stationary phases for CEC, monolithic stationary phase has been regarded as the most suitable stationary phase for CEC because of its simple preparation, the elimination of frits, and its excellent performance. In this chapter, procedures for preparing CEC monolithic columns with an improved configuration, in which there are stationary phases at both sides of detection window and no stationary phase at detection window, are presented. The separation of acidic and basic compounds on such monolithic columns is used as an example to demonstrate CEC separation protocol. Additionally, an on-line concentration technique in CEC is presented. As a result of the coexistence of stationary phase and electric field in a CEC column, it is possible to employ chromatographic zone sharpening and field-amplified sample stacking effects simultaneously to improve CEC detection sensitivity.
Altmetric
Additional Metrics?
[➜Log in]
Edit extra informations Login
Publication type Article: Journal article
Document type Scientific Article
Editors Schmitt-Kopplin, P.
Keywords Capillary electrochromatography; separation mode; mobile phase; stationary phase; monolithic column; electroseparation; electroosmotic flow; on-line concentration; chromatographic zone sharpening effect; field amplified sample stacking effect
ISSN (print) / ISBN 1064-3745
e-ISSN 1940-6029
Conference Title Capillary Electrophoresis: Methods and Protocols
Journal Methods in Molecular Biology
Quellenangaben Volume: 384, Issue: , Pages: 751-769 Article Number: , Supplement: ,
Series Methods in Molecular Biology
Publisher Springer
Publishing Place Berlin [u.a.]
Reviewing status Peer reviewed
Institute(s) Institute of Ecological Chemistry (IOEC)
Research Unit BioGeoChemistry and Analytics (BGC)
Institute of Virology (VIRO)