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Zipplies, J.K.* ; Hauck, S.M. ; Schoeffmann, S. ; Amann, B.* ; Stangassinger, M.* ; Ueffing, M. ; Deeg, C.A.*

Serum PEDF levels are decreased in a spontaneous animal model for human autoimmune uveitis.

J. Proteome Res. 8, (Sp. Iss. SI), 992-998 (2009)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Identification of biomarkers is of critical relevance toward improving diagnosis and therapy of autoimmune disorders. Serum markers are a desirable choice as sera are easily accessible and the development of assays for routine clinical detection prompts feasible. Autoimmune uveitis, a recurrent disease affecting the eye, is characterized by returning inflammatory attacks of the inner eye followed by variable periods of quiescent stages. Spontaneous equine recurrent uveitis (ERU) is the equine equivalent and serves as a model for the human disease. To identify potential biomarker candidates, we first systematically compared the proteomes of individual ERU cases with healthy controls by proteomic profiling using 2-D difference-gel-electrophoresis (2-D DIGE) followed by tandem mass spectrometry. A total of seven differentially expressed proteins were identified. Besides the upregulation of IgG and the significant lower expression of albumin, Antithrombin III, and Vitamin D binding protein, we found complement components C1q and C4, to be downregulated in uveitic state. Interestingly, Pigment epithelium-derived factor (PEDF), a marker already detected by 2DE differential proteome analysis in ERU target tissues, vitreous and retina, was found to be also significantly downregulated in sera. The lower expression of PEDF in sera of horses with uveitis could be verified in a cohort of 116 ERU cases and 115 healthy controls. Our findings of a significant lower PEDF expression in ERU cases also in the periphery of the eye proves PEDF as a promising uveitis biomarker.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Pigment epithelium derived factor (PEDF); serum; biomarker; Fluorescence two-dimensional difference gel electrophoresis; uveitis; difference gel-electrophoresis; epithelium-derived factor; d-binding protein; equine recurrent uveitis; vitamin-d; retinal autoantigens; mass-spectrometry; s-antigen; disease; gene
Language
Publication Year 2009
HGF-reported in Year 2009
ISSN (print) / ISBN 1535-3893
e-ISSN 1535-3907
Journal Journal of Proteome Research
Quellenangaben Volume: 8, Issue: 2, Pages: 992-998, Article Number: , Supplement: (Sp. Iss. SI)
Publisher American Chemical Society (ACS)
Reviewing status Peer reviewed
Institute(s) CF Metabolomics & Proteomics (CF-MPC)
POF-Topic(s) 30203 - Molecular Targets and Therapies
Research field(s) Enabling and Novel Technologies
PSP Element(s) G-505700-001
DOI 10.1021/pr800694y
Scopus ID 61849086258
PubMed ID 19113885
Erfassungsdatum 2009-12-31
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