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Open Access Green as soon as Postprint is submitted to ZB.
Effects of genetically modified potatoes with increased zeaxanthin content on the abundance and diversity of rhizobacteria with in vitro antagonistic activity do not exceed natural variability among cultivars.
Plant Soil 326, 437-452 (2010)
To assess potential effects of genetically modified (GM) potatoes on the abundance and diversity of rhizobacteria with in vitro antagonistic activity in relation to natural variability among cultivars, two GM potato lines accumulating the carotenoid zeaxanthin in their tubers, the parental cultivar and four additional commercial cultivars were planted at two field sites in Germany. Rhizosphere samples were taken at three developmental stages of the plants. A total of 3,985 bacteria isolated from the rhizosphere were screened for their in vitro antagonistic activity towards Rhizoctonia solani, Verticillium dahliae and Phytophthora infestans using a dual-culture assay. Genotypic characterisation, 16S rRNA gene sequencing and antifungal metabolite analysis was performed to characterize the 595 antagonists obtained. The 16S rRNA gene-based identification of in vitro antagonists revealed strong site-dependent differences in their taxonomic composition. This study showed that the site was the overriding factor determining the proportion and diversity of antagonists from the rhizosphere of potato while the effect of the genetic modification on the proportion of antagonists obtained did not exceed natural variability among the five commercial cultivars tested.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Genetically modified potatoes; In vitro antagonists; BOX; 16S rRNA gene
ISSN (print) / ISBN
0032-079X
e-ISSN
1573-5036
Journal
Plant and Soil
Quellenangaben
Volume: 326,
Issue: 1-2,
Pages: 437-452
Publisher
Springer
Reviewing status
Peer reviewed
Institute(s)
Institute of Soil Ecology (IBOE)