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Open Access Green as soon as Postprint is submitted to ZB.
Construction and isolation of recombinant MVA.
Methods Mol. Biol. 269, 77-99 (2004)
Modified vaccinia virus Ankara (MVA) is a valuable tool for the expression of recombinant genes used for such purposes as the study of protein functions or characterization of cellular and humoral immune responses. A major advantage of MVA is its clear safety record, and it can be handled under biosafety level 1 conditions. Despite its replication deficiency in human and most mammalian cells, MVA provides high-level gene expression and has proven to be immunogenic when delivering heterologous antigens in animals and humans. This chapter provides state-of-the-art protocols for generation, plaque isolation, molecular characterization, as well as amplification and purification of MVA vector viruses to obtain recombinant viruses for further evaluation.
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Publication type
Article: Journal article
Document type
Scientific Article
Editors
Isaacs, S.N.*
Keywords
poxvirus; modified vaccinia virus Ankara (MVA); recombinant vaccine
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Conference Title
Vaccinia Virus and Poxvirology: Methods and Protocols
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 269,
Pages: 77-99
Series
Methods in Molecular Biology
Publisher
Springer
Publishing Place
Berlin [u.a.]
Reviewing status
Peer reviewed
Institute(s)
Institute of Virology (VIRO)