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Lubeseder-Martellato, C. ; Guenzi, E. ; Jörg, A. ; Töpolt, K. ; Naschberger, E. ; Kremmer, E. ; Zietz, C.* ; Tschachler, E.* ; Hutzler, P. ; Schwemmle, M.* ; Matzen, K. ; Grimm, Th. ; Ensoli, B.*

Guanylate-Binding Protein-1 Expression is Selectively Induced by Inflammatory Cytokines and is an Activation Marker of Endothelial Cells during Inflammatory Diseases.

Am. J. Pathol. 161, 1749-1759 (2002)
DOI
Open Access Green as soon as Postprint is submitted to ZB.
During angiogenesis and inflammatory processes, endothelial cells acquire different activation phenotypes, whose identification may help in understanding the complex network of angiogenic and inflammatory interactions in vivo. To this goal we investigated the expression of the human guanylate-binding protein (GBP)-1 that is highly induced by inflammatory cytokines, (ICs) and, therefore, may characterize IC-activated cells. Using a new rat monoclonal antibody raised against GBP-1, we show that GBP-1 is a cytoplasmic protein and that its expression in endothelial cells is selectively induced by interferon-gamma, interleukin-1alpha, interleukin-1beta, or tumor necrosis factor-a, but not by other cytokines, chemokines, or growth factors. Moreover, we found that GBP-1 expression is highly associated with vascular endothelial cells as confirmed by the simultaneous detection of GBP-1 and the endothelial cell-associated marker CD31 in a broad range of human tissues. Notably, GBP-1 expression was undetectable in the skin, but it was highly induced in vessels of skin diseases with a high-inflammatory component including psoriasis, adverse drug reactions, and Kaposi's sarcoma. These results indicate that GBP-1 is a novel cellular activation marker that characterizes the IC-activated phenotype of endothelial cells.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords ANGIOGENESIS IN-VITRO; TUMOR-NECROSIS-FACTOR; GAMMA-INTERFERON; GROWTH-FACTOR; KAPOSIS-SARCOMA; ADHESION MOLECULE-1; SPINDLE CELLS; VASCULAR-PERMEABILITY; NUCLEOTIDE-BINDING; GENE-EXPRESSION
ISSN (print) / ISBN 0002-9440
e-ISSN 1525-2191
Journal American Journal of Pathology, The
Quellenangaben Volume: 161, Issue: 5, Pages: 1749-1759 Article Number: , Supplement: ,
Publisher Elsevier
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Pathology (PATH)
Institute of Molecular Immunology (IMI)
Institute of Virology (VIRO)