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Open Access Green as soon as Postprint is submitted to ZB.
Targeted metabolomics of dried blood spot extracts.
Chromatographia 76, 1295-1305 (2013)
Dried blood spot (DBS) samples are already successfully used in newborn screening and pharmacological analyses. The application of DBS matrix to further metabolomic methods will considerably extend the analytical options for the diagnostics of metabolic diseases. We present an MS/MS based method for the simultaneous extraction and quantification of 188 metabolites from dried blood spots. We provide a sensitive and reproducible method that adapts the Absolute IDQ™ p180 kit of Biocrates to the DBS matrix for the quantification of metabolites of different substance classes including amino acids, biogenic amines, free carnitine, acylcarnitines, hexoses, glycerophospholipids, lysophosphatidylcholines, phosphatidylcholines, and sphingolipids.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Dried blood spots; Lipids; Mass spectrometry; Metabolite quantification; Targeted metabolomics; Tandem Mass-spectrometry ; Amino-acids ; Validation ; Acylcarnitines ; Biomarkers ; Stability ; Hplc
ISSN (print) / ISBN
0009-5893
e-ISSN
1612-1112
Journal
Chromatographia
Quellenangaben
Volume: 76,
Issue: 19-20,
Pages: 1295-1305
Publisher
Springer
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Molekulare Endokrinologie und Metabolismus (MEM)
Institute of Experimental Genetics (IEG)
Institute of Experimental Genetics (IEG)