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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
The impact of blood on liver metabolite profiling - a combined metabolomic and proteomic approach.
Biomed. Chromatogr. 28, 231-240 (2014)
Metabolomics has entered the well-established omic sciences as it is an indispensable information resource to achieve a global picture of biological systems. The aim of the present study was to estimate the influence of blood removal from mice liver as part of sample preparation for metabolomic and proteomic studies. For this purpose, perfused mice liver tissue (i.e. with blood removed) and unperfused mice liver tissue (i.e. containing blood) were compared by two-dimensional gas chromatography time of flight mass spectrometry (GC × GC-TOFMS) for the metabolomic part, and by liquid chromatography tandem mass spectrometry (LC-MS/MS) for the proteomic part. Our data showed significant differences between the unperfused and perfused liver tissue samples. Furthermore, we also observed an overlap of blood and tissue metabolite profiles in our data, suggesting that the perfusion of liver tissue prior to analysis is beneficial for an accurate metabolic profile of this organ
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
blood; gas chromatography mass spectrometry; liquid chromatography tandem mass spectrometry; metabolomics; mice liver perfusion; proteomics; 2-dimensional Gas-chromatography; Flight Mass-spectrometry; Fatty Liver; Tissue-extracts; Mice; Plasma; Time; Metabonomics; Steatosis; Disease
ISSN (print) / ISBN
0269-3879
e-ISSN
1099-0801
Zeitschrift
Biomedical Chromatography
Quellenangaben
Band: 28,
Heft: 2,
Seiten: 231-240
Verlag
Wiley
Verlagsort
Hoboken
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed