OpenSSL SSL_connect: Connection reset by peer in connection to v2.sherpa.ac.uk:443 PuSH - Publication Server of Helmholtz Zentrum München: The CR4 region of EBNA2 convers viability of Epstein-Barr virus-transformed B cells by CBF1-independent signalling.

PuSH - Publication Server of Helmholtz Zentrum München

Grabusic, K. ; Maier, S. ; Hartmann, A. ; Mantik, A. ; Hammerschmidt, W. ; Kempkes, B.

The CR4 region of EBNA2 convers viability of Epstein-Barr virus-transformed B cells by CBF1-independent signalling.

J. Gen. Virol. 87, 3169-3176 (2006)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
The Epstein-Barr virus (EBV) nuclear antigen 2 (EBNA2) gene product is the key regulator of the latent genes of EBV and essential for EBV-mediated transformation of human primary B cells. Viral mutants were constructed carrying a deletion of the EBNA2 conserved region 4 (CR4). Primary resting B cells infected with the DeltaCR4-EBNA2 mutant virus were dramatically impaired for B cell transformation. Lymphoblastoid cell lines (LCLs) established with this mutant EBV revealed a prolonged population doubling time when cells were cultivated at low cell densities, which are not critical for wild-type-infected cells. Low-level spontaneous cell death occurred when the cells were cultivated at suboptimal cell densities. The phenotype of B cells and LCLs infected with the DeltaCR4-EBNA2 mutant virus indicated that the CR4 region of EBNA2 specifically contributes to the viability of the cells rather than affecting cell division rates.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
ISSN (print) / ISBN 0022-1317
e-ISSN 1465-2099
Journal Journal of General Virology
Quellenangaben Volume: 87, Issue: 11, Pages: 3169-3176 Article Number: , Supplement: ,
Publisher Society for General Microbiology
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Research Unit Gene Vector (AGV)