PuSH - Publication Server of Helmholtz Zentrum München: Hyaluronan carried by tumor-derived microvesicles induces IL-10 production in classical CD14<sup>++</sup> CD16<sup>-</sup> monocytes via PI3K/Akt/mTOR-dependent signalling pathway.

Information

Clues to quality of journals

Open Access Policy of Helmholtz Association 2016

CC Licencences

Publication Metrics

Navigation

Home

Deutsch

EVA: Electronic Publishing

New EVA Application

Research

Advanced Search

Browse by ...

... HMGU-Authors/Consortia

... Organizational Structure

... Journal

... Publication Type

... Research Data

... Arbeitsgruppen

... Publication Year

Publication overview

Statistics

Statistics (last 5 years)

OA Publications

Publish

Submit Publication

Import publication from...

...EVA

Report missing publication

Highlights

Support & Contact

Contact persons

Help

Data protection

Helmholtz Open Science

JANE Journal Estimator

SHERPA/RoMEO

DOAJ

Export:

Text

Endnote (RIS) BIB

BibTeX

Lenart, M.* ; Rutkowska-Zapala, M.* ; Baj-Krzyworzeka, M.* ; Szatanek, R.* ; Węglarczyk, K.* ; Smallie, T.* ; Ziegler-Heitbrock, L. ; Zembala, M.* ; Siedlar, M.*

Hyaluronan carried by tumor-derived microvesicles induces IL-10 production in classical CD14⁺⁺ CD16^- monocytes via PI3K/Akt/mTOR-dependent signalling pathway.

Immunobiology 222, 1-10 (2015)

DOI

PMC

Open Access Gold as soon as Publ. Version/Full Text is submitted to ZB.

Abstract
Metrics
Extra information

Tumor-derived microvesicles (TMV) can mimic effects of tumor cells leading to an increased anti-inflammatory cytokine production, such as interleukin 10 (IL-10), by tumor-infiltrating monocytes and macrophages. Yet, the mechanism of IL-10 induction by TMV in monocytes remains unclear. The co-incubation of TMV derived from the human pancreas carcinoma cell line (HPC-4) with human monocytes resulted in a nearly 30-fold increase in IL-10 protein production. This effect operates at the level of transcription since monocytes transduced with an adenovirus containing IL-10-promoter luciferase reporter gene showed a 5-fold induction of luciferase activity after treatment with TMV. Since tumor cells can express hyaluronan (HA), which participates in tumor invasion and metastases, we have tested its effect on IL-10 expression. We showed that HA at the concentration of 100μg/ml induces IL-10 protein expression and the IL-10 promoter activation in monocytes. Moreover, hyaluronidase treatment of TMV reduced IL-10 protein production by 50% and promoter activity by 40%. Inhibitors of the PI3K/Akt/mTOR pathway reduced both, TMV-induced IL-10 promoter activity and protein production, and the same was observed in monocytes when stimulated by HPC-4 cells or HA. Inhibition of PI3K activity down-regulated phosphorylation of the Akt and (to a lesser extent) mTOR proteins in monocytes following TMV or HA stimulation. When comparing monocyte subsets, TMV induced IL-10 protein and mRNA synthesis only in classical CD14(++)CD16(-) but not in CD16-positive monocytes. Our data show that TMV induce IL-10 synthesis in human classical monocytes via HA, which, in turn, activates the PI3K/Akt/mTOR pathway.

Impact Factor

Scopus SNIP

Web of Science
Times Cited

Scopus
Cited By

Altmetric

3.044

0.866

PuSH - Publication Server of Helmholtz Zentrum München

Hyaluronan carried by tumor-derived microvesicles induces IL-10 production in classical CD14++ CD16- monocytes via PI3K/Akt/mTOR-dependent signalling pathway.

Hyaluronan carried by tumor-derived microvesicles induces IL-10 production in classical CD14⁺⁺ CD16^- monocytes via PI3K/Akt/mTOR-dependent signalling pathway.