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Ma, X. ; Prakash, J. ; Ruscitti, F.* ; Glasl, S. ; Stellari, F.F.* ; Villetti, G.* ; Ntziachristos, V.

Assessment of asthmatic inflammation using hybrid fluorescence molecular tomography-X-ray computed tomography.

J. Biomed. Opt. 21:15009 (2016)

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Nuclear imaging plays a critical role in asthma research but is limited in its readings of biology due to the short-lived signals of radio-isotopes. We employed hybrid fluorescence molecular tomography (FMT) and x-ray computed tomography (XCT) for the assessment of asthmatic inflammation based on resolving cathepsin activity and matrix metalloproteinase activity in dust mite, ragweed, and Aspergillus species-challenged mice. The reconstructed multimodal fluorescence distribution showed good correspondence with ex vivo cryosection images and histological images, confirming FMT-XCT as an interesting alternative for asthma research.

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