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Open Access Green as soon as Postprint is submitted to ZB.
Objective, comparative assessment of the penetration depth of temporal-focusing microscopy for imaging various organs.
J. Biomed. Opt. 20:61107 (2015)
Temporal focusing is a technique for performing axially resolved widefield multiphoton microscopy
with a large field of view. Despite significant advantages over conventional point-scanning multiphoton microscopy
in terms of imaging speed, the need to collect the whole image simultaneously means that it is expected to
achieve a lower penetration depth in common biological samples compared to point-scanning. We assess the
penetration depth using a rigorous objective criterion based on the modulation transfer function, comparing it to
point-scanning multiphoton microscopy. Measurements are performed in a variety of mouse organs in order to
provide practical guidance as to the achievable penetration depth for both imaging techniques. It is found that
two-photon scanning microscopy has approximately twice the penetration depth of temporal-focusing microscopy,
and that penetration depth is organ-specific; the heart has the lowest penetration depth, followed by the
liver, lungs, and kidneys, then the spleen, and finally white adipose tissue.
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Publication type
Article: Journal article
Document type
Scientific Article
ISSN (print) / ISBN
1083-3668
e-ISSN
1560-2281
Journal
Journal of Biomedical Optics
Quellenangaben
Volume: 20,
Issue: 6,
Article Number: 61107
Publisher
SPIE
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Helmholtz Pioneer Campus (HPC)