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A simplified method for bronchoalveolar lavage in mice by orotracheal intubation avoiding tracheotomy.
BioTechniques 71, 534-537 (2021)
Bronchoalveolar lavage (BAL) represents an important method to sample immune cells and soluble substances from the lungs of humans and animals suffering from respiratory disease. The mouse is the most commonly used model organism to study lung disease. Performing BAL in mice is difficult due to their small size and the currently used method requires tracheotomy, a complex and time-consuming procedure. Here, we describe a simple alternative procedure that avoids this step. To perform the BAL, a rigid, olive tip cannula is inserted from the mouth into the trachea under visual inspection. This novel method requires minimal training, is simple, fast, inexpensive and should be useful for researchers studying mouse models of human lung disease.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Bronchoalveolar Lavage (bal) ; Mice ; Mouse Lung Disease Models ; Noninvasive Orotracheal Intubation ; Olive Tip Cannula ; Vaccinia Virus; Mouse Models; Genome
ISSN (print) / ISBN
0736-6205
e-ISSN
1940-9818
Journal
BioTechniques / Protocol Guide
Quellenangaben
Volume: 71,
Issue: 4,
Pages: 534-537
Publisher
Informa Life Sciences Publ.
Publishing Place
New York, NY
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Virology (VIRO)