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From RNA-seq to large-scale genotyping - genomics resources for rye (Secale cereale L.).
BMC Plant Biol. 11:131 (2011)
BACKGROUND: The improvement of agricultural crops with regard to yield, resistance and environmental adaptation is a perpetual challenge for both breeding and research. Exploration of the genetic potential and implementation of genome-based breeding strategies for efficient rye (Secale cereale L.) cultivar improvement have been hampered by the lack of genome sequence information. To overcome this limitation we sequenced the transcriptomes of five winter rye inbred lines using Roche/454 GS FLX technology. RESULTS: More than 2.5 million reads were assembled into 115,400 contigs representing a comprehensive rye expressed sequence tag (EST) resource. From sequence comparisons 5,234 single nucleotide polymorphisms (SNPs) were identified to develop the Rye5K high-throughput SNP genotyping array. Performance of the Rye5K SNP array was investigated by genotyping 59 rye inbred lines including the five lines used for sequencing, and five barley, three wheat, and two triticale accessions. A balanced distribution of allele frequencies ranging from 0.1 to 0.9 was observed. Residual heterozygosity of the rye inbred lines varied from 4.0 to 20.4% with higher average heterozygosity in the pollen compared to the seed parent pool. CONCLUSIONS: The established sequence and molecular marker resources will improve and promote genetic and genomic research as well as genome-based breeding in rye.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
EST resource; next generation sequencing; Secale cereale L.; Rye5K SNP array; single nucleotide polymorphisms; MOLECULAR MARKERS; DRAFT SEQUENCE; MESSENGER-RNA; GENETIC-MAP; TRANSCRIPTOME; BARLEY; DISCOVERY; MICROSATELLITE; ANNOTATION; DATABASE
ISSN (print) / ISBN
1471-2229
e-ISSN
1471-2229
Journal
BMC Plant Biology
Quellenangaben
Volume: 11,
Article Number: 131
Publisher
BioMed Central
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Reviewing status
Peer reviewed