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Meinhold, M.* ; Verbrugge, S.* ; Shi, A.* ; Schönfelder, M.* ; Becker, L. ; Jaspers, R.T.* ; Zammit, P.S.* ; Wackerhage, H.*

Yap/Taz activity is associated with increased expression of phosphoglycerate dehydrogenase that supports myoblast proliferation.

Cell Tissue Res. 395, 271–283 (2024)
DOI PMC
Creative Commons Lizenzvertrag
In skeletal muscle, the Hippo effector Yap promotes satellite cell, myoblast, and rhabdomyoblast proliferation but prevents myogenic differentiation into multinucleated muscle fibres. We previously noted that Yap drives expression of the first enzyme of the serine biosynthesis pathway, phosphoglycerate dehydrogenase (Phgdh). Here, we examined the regulation and function of Phgdh in satellite cells and myoblasts and found that Phgdh protein increased during satellite cell activation. Analysis of published data reveal that Phgdh mRNA in mouse tibialis anterior muscle was highly expressed at day 3 of regeneration after cardiotoxin injection, when markers of proliferation are also robustly expressed and in the first week of synergist-ablated muscle. Finally, siRNA-mediated knockdown of PHGDH significantly reduced myoblast numbers and the proliferation rate. Collectively, our data suggest that Phgdh is a proliferation-enhancing metabolic enzyme that is induced when quiescent satellite cells become activated.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Myoblast ; Phgdh ; Phosphoglycerate Dehydrogenase ; Skeletal Muscle ; Taz ; Yap; Skeletal-muscle; Gene-expression; Satellite Cells; 3-phosphoglycerate Dehydrogenase; Serine Synthesis; Hippo Pathway; Reveals; Yap; Metabolism; Drosophila
ISSN (print) / ISBN 0044-3794
e-ISSN 1432-0878
Zeitschrift Cell and Tissue Research
Quellenangaben Band: 395, Heft: , Seiten: 271–283 Artikelnummer: , Supplement: ,
Verlag Springer
Verlagsort One New York Plaza, Suite 4600, New York, Ny, United States
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Experimental Genetics (IEG)
Förderungen Technische Universitt Mnchen (1025)