Open Access Green: Postprint online verfügbar 04/2025
möglich sobald bei der ZB eingereicht worden ist.
Controlling the sound of light: photoswitching optoacoustic imaging.
Nat. Methods, DOI: 10.1038/s41592-024-02396-2 (2024)
DOI
PMC
Optoacoustic (photoacoustic) imaging advances allow high-resolution optical imaging much deeper than optical microscopy. However, while label-free optoacoustics have already entered clinical application, biological imaging is in need of ubiquitous optoacoustic labels for use in ways that are similar to how fluorescent proteins propelled optical microscopy. We review photoswitching advances that shine a new light or, in analogy, 'bring a new sound' to biological optoacoustic imaging. Based on engineered labels and novel devices, switching uses light or other energy forms and enables signal modulation and synchronous detection for maximizing contrast and detection sensitivity over other optoacoustic labels. Herein, we explain contrast enhancement in the spectral versus temporal domains and review labels and key concepts of switching and their properties to modulate optoacoustic signals. We further outline systems and applications and discuss how switching can enable optoacoustic imaging of cellular or molecular contrast at depths and resolutions beyond those of other optical methods.
Altmetric
Weitere Metriken?
Zusatzinfos bearbeiten
[➜Einloggen]
Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Review
Schlagwörter
Infrared Fluorescent Proteins; Deep Tissue; Tomography; Microscopy; Mesoscopy
ISSN (print) / ISBN
1548-7091
e-ISSN
1548-7105
Zeitschrift
Nature Methods
Verlag
Nature Publishing Group
Verlagsort
New York, NY
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Förderungen
European Research Council (ERC)
Deutsche Forschungsgemeinschaft
European Union
Deutsche Forschungsgemeinschaft
European Union