OpenSSL SSL_connect: Connection reset by peer in connection to v2.sherpa.ac.uk:443 PuSH - Publikationsserver des Helmholtz Zentrums München: Model-based temporal unmixing towards quantitative photo-switching optoacoustic tomography.

PuSH - Publikationsserver des Helmholtz Zentrums München

Liu, Y.* ; Chuah, J.* ; Huang, Y. ; Stiel, A.-C. ; Unser, M.* ; Dong, J.*

Model-based temporal unmixing towards quantitative photo-switching optoacoustic tomography.

Opt. Express. 33, 6216-6227 (2025)
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Optoacoustic (OA) imaging combined with reversibly photoswitchable proteins has emerged as a promising technology for the high-sensitivity and multiplexed imaging of cells in live tissues in preclinical research. Through carefully designed illumination schedules of ON and OFF laser pulses, the resulting OA signal is a multiplex of different reporter species and the background. We propose a model-based variational framework to computationally unmix and image different species of photo-switching reporters using optoacoustic tomography. It is based on a detailed mathematical description of the photo-switching mechanism, which models how relevant physical parameters such as the kinetic constants and light fluence impact the switching signal. We introduce an algorithm that operates on images, as opposed to traditional pixelwise approaches. It takes the form of an iterative inversion combined with tailored ℓ1 and total-variation regularization to increase the robustness to noise and to improve the unmixing quality. We show that our method can disentangle multiple spatially overlapping labels and recover continuous maps of quantities of interest on controlled phantoms and mice experiments.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
ISSN (print) / ISBN 1094-4087
e-ISSN 1094-4087
Zeitschrift Optics Express
Quellenangaben Band: 33, Heft: 3, Seiten: 6216-6227 Artikelnummer: , Supplement: ,
Verlag Optical Society of America (OSA)
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biological and Medical Imaging (IBMI)