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Frohn, A.* ; Eberl, H.C.* ; Stöhr, J.* ; Glasmacher, E. ; Rüdel, S.* ; Heissmeyer, V. ; Mann, M.* ; Meister, G.*

Dicer-dependent and -independent Argonaute2 protein interaction networks in mammalian cells.

Mol. Cell. Proteomics 11, 1442-1456 (2012)
Verlagsversion Volltext DOI PMC
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Argonaute (Ago) proteins interact with small regulatory RNAs such as microRNAs (miRNAs) and facilitate gene-silencing processes. miRNAs guide Ago proteins to specific mRNAs leading to translational silencing or mRNA decay. In order to understand the mechanistic details of miRNA function, it is important to characterize Ago protein interactors. Although several proteomic studies have been performed, it is not clear how the Ago interactome changes on miRNA or mRNA binding. Here, we report the analysis of Ago protein interactions in miRNA-containing and miRNA-depleted cells. Using stable isotope labeling in cell culture in conjunction with Dicer knock out mouse embryonic fibroblasts, we identify proteins that interact with Ago2 in the presence or the absence of Dicer. In contrast to our current view, we find that Ago-mRNA interactions can also take place in the absence of miRNAs. Our proteomics approach provides a rich resource for further functional studies on the cellular roles of Ago proteins.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Embryonic Stem-cells ; Quantitative Mass-spectrometry ; Messenger-rna Decay ; Translational Repression ; Microrna Biogenesis ; Stress Granules ; Mirna Targets ; Complexes ; Proteomics ; Binding
Sprache englisch
Veröffentlichungsjahr 2012
HGF-Berichtsjahr 2012
ISSN (print) / ISBN 1535-9476
e-ISSN 1535-9484
Zeitschrift Molecular and Cellular Proteomics
Quellenangaben Band: 11, Heft: 11, Seiten: 1442-1456 Artikelnummer: , Supplement: ,
Verlag American Society for Biochemistry and Molecular Biology
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Molecular Immunology (IMI)
Institute of Diabetes and Obesity (IDO)
POF Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
30502 - Diabetes: Pathophysiology, Prevention and Therapy
Forschungsfeld(er) Immune Response and Infection
Helmholtz Diabetes Center
PSP-Element(e) G-501792-001
G-553100-001
PubMed ID 22918229
DOI 10.1074/mcp.M112.017756
WOS ID WOS:000313277100026
Scopus ID 84869237206
Erfassungsdatum 2012-12-06
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