PuSH - Publikationsserver des Helmholtz Zentrums München: Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes.

Informationen

Hinweise zu Qualitätskriterien von Zeitschriften

Open-Access-Richtlinie der Helmholtz-Gemeinschaft 2016

CC Licencences

Metriken für Publikationen

Navigation

Startseite

English

EVA: Veröffentlichen

Neuer EVA Antrag

Recherche

Erweiterte Suche

Durchblättern nach ...

... HMGU-Autoren/Konsortien

... Organisationsstruktur

... Zeitschriften

... Publikationstypen

... Forschungsdaten

... Arbeitsgruppen

... Erscheinungsjahr

Publikationen im Überblick

Statistik

HGF Fortschrittsbericht

OA Publikationen

Eintragen

Neue Publikation eintragen

Neue Publikation holen aus...

...EVA

Fehlende Publikation melden

Highlights

Suche

Hilfe & Kontakt

Ansprechpartner

Hilfe

Datenschutz

Helmholtz Open Science

Bibliometrische Indikatoren

SHERPA/RoMEO

DOAJ

Export:

Text

Endnote (RIS) BIB

BibTeX

Klionsky, D.J.* ; Abeliovich, H.* ; Agostinis, P.* ; Agrawal, D.K.* ; Aliev, G.* ; Askew, D.S.* ; Baba, M.* ; Baehrecke, E.H.* ; Bahr, BA.* ; Ballabio, A.* ; Bamber, BA.* ; Bassham, D.C.* ; Bergamini, E.* ; Bi, X.* ; Biard-Piechaczyk, M.* ; Blum, J.S.* ; Bredesen, D.E.* ; Brodsky, J.L.* ; Brumell, J.H.* ; Brunk, U.T.* ; Bursch, W.* ; Camougrand, N.* ; Cebollero, E.* ; Cecconi, F.* ; Chen, Y.* ; Chin, L.S.* ; Choi, A.* ; Chu, C.T.* ; Chung, J.* ; Clarke, P.G.* ; Clark, R.S.* ; Clarke, S.G.* ; Clavé, C.* ; Cleveland, J.L.* ; Codogno, P.* ; Colombo, M.I.* ; Coto-Montes, A.* ; Cregg, J.M.* ; Cuervo, A.M. ; Debnath, J.* ; Demarchi, F.* ; Dennis, P.B.* ; Dennis, P.A.* ; Deretic, V.* ; Devenish, R.J.* ; Di, Sano, F.* ; Dice, J.F.* ; Difiglia, M.* ; Dinesh-Kumar, S.* ; Distelhorst, C.W.* ; Djavaheri-Mergny, M.* ; Dorsey, F.C.* ; Dröge, W.* ; Dron, M.* ; Dunn, W.A, Jr.* ; Duszenko, M.* ; Eissa, N.T.* ; Elazar, Z.* ; Esclatine, A.* ; Eskelinen, E.L.* ; Fésüs, L.* ; Finley, KD.* ; Fuentes, J.M.* ; Fueyo, J.* ; Fujisaki, K.* ; Galliot, B.* ; Gao, F.B.* ; Gewirtz, D.A.* ; Gibson, SB.* ; Gohla, A.* ; Goldberg, A.L.* ; González, R.* ; González-Estévez, C.* ; Gorski, S.* ; Gottlieb, R.A.* ; Häussinger, D.* ; He, Y.W.* ; Heidenreich, K.* ; Hill, J.A.* ; Høyer-Hansen, M.* ; Hu, X.* ; Huang, W.P.* ; Iwasaki, A.* ; Jäättelä, M.* ; Jackson, W.T.* ; Jiang, X.* ; Jin, S.* ; Johansen, T.* ; Jung, J.U.* ; Kadowaki, M.* ; Kang, C.* ; Kelekar, A.* ; Kessel, D.H.* ; Kiel, J.A.* ; Kim, H.P.* ; Kimchi, A.* ; Kinsella, T.J.* ; Kiselyov, K.* ; Kitamoto, K.* ; Knecht, E.* ; Komatsu, M.* ; Kominami, E.* ; Kondo, S.* ; Kovács, A.L.* ; Kroemer, G.* ; Kuan, C.Y.* ; Kumar, R.* ; Kundu, M.* ; Landry, J.* ; Laporte, M.* ; Le, W.* ; Lei, H.Y.* ; Lenardo, M.J.* ; Levine, B.* ; Lieberman, A.* ; Lim, K.L.* ; Lin, F.C.* ; Liou, W.* ; Liu, L.F.* ; Lopez-Berestein, G.* ; López-Otín, C.* ; Lu, B.* ; Macleod, K.F.* ; Malorni, W.* ; Martinet, W.* ; Matsuoka, K.* ; Mautner, J. ; Meijer, A.J.* ; Meléndez, A.* ; Michels, P.* ; Miotto, G.* ; Mistiaen, W.P.* ; Mizushima, N.* ; Mograbi, B.* ; Monastyrska, I.* ; Moore, M.N.* ; Moreira, P.I.* ; Moriyasu, Y.* ; Motyl, T.* ; Münz, C.* ; Murphy, L.O.* ; Naqvi, N.I.* ; Neufeld, T.P.* ; Nishino, I.* ; Nixon, R.A.* ; Noda, T.* ; Nürnberg, B.* ; Ogawa, M.* ; Oleinick, NL.* ; Olsen, LJ.* ; Ozpolat, B.* ; Paglin, S.* ; Palmer, G.E.* ; Papassideri, I.* ; Parkes, M.* ; Perlmutter, D.H.* ; Perry, G.* ; Piacentini, M.* ; Pinkas-Kramarski, R.* ; Prescott, M.* ; Proikas-Cezanne, T.* ; Raben, N.* ; Rami, A.* ; Reggiori, F.* ; Rohrer, B.* ; Rubinsztein, D.C.* ; Ryan, K.M.* ; Sadoshima, J.* ; Sakagami, H.* ; Sakai, Y.* ; Sandri, M.* ; Sasakawa, C.* ; Sass, M.* ; Schneider, C.* ; Seglen, P.O.* ; Seiliez, I.* ; Seleverstov, O.* ; Settleman, J.* ; Shacka, J.J.* ; Shapiro, I.M.* ; Sibirny, A.* ; Silva-Zacarin, E.C.* ; Simon, H.U.* ; Simone, C.* ; Simonsen, A.* ; Smith, M.A.* ; Spanel-Borowski, K.* ; Srinivas, V.* ; Steeves, M.* ; Stenmark, H.* ; Stromhaug, P.E.* ; Subauste, C.S.* ; Sugimoto, S.* ; Sulzer, D.* ; Suzuki, T.* ; Swanson, M.S.* ; Tabas, I.* ; Takeshita, F.* ; Talbot, N.J.* ; Tallóczy, Z.* ; Tanaka, K.* ; Tanida, I.* ; Taylor, G.S.* ; Taylor, J.P.* ; Terman, A. ; Tettamanti, G.* ; Thompson, C.B.* ; Thumm, M.* ; Tolkovsky, A.M.* ; Tooze, S.A.* ; Truant, R.* ; Tumanovska, L.V.* ; Uchiyama, Y.* ; Ueno, T.* ; Uzcátegui, N.L.* ; van der Klei, I.* ; Vaquero, E.C.* ; Vellai, T.* ; Vogel, M.W.* ; Wang, H.G. ; Webster, P.* ; Wiley, J.W.* ; Xi, Z.* ; Xiao, G.* ; Yahalom, J.* ; Yang, J.M.* ; Yap, G.* ; Yin, X.M.* ; Yoshimori, T.* ; Yu, L.* ; Yue, Z.* ; Yuzaki, M.* ; Zabirnyk, O.* ; Zheng, X.* ; Zhu, X.* ; Deter, R.L.*

Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes.

Autophagy 4, 151-175 (2008)

Verlagsversion

Erratum

PMC

Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.

Abstract
Metriken
Zusatzinfos

Research in autophagy continues to accelerate,(1) and as a result many new scientists are entering the field. Accordingly, it is important to establish a standard set of criteria for monitoring macroautophagy in different organisms. Recent reviews have described the range of assays that have been used for this purpose.(2,3) There are many useful and convenient methods that can be used to monitor macroautophagy in yeast, but relatively few in other model systems, and there is much confusion regarding acceptable methods to measure macroautophagy in higher eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers of autophagosomes versus those that measure flux through the autophagy pathway; thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from fully functional autophagy that includes delivery to, and degradation within, lysosomes (in most higher eukaryotes) or the vacuole (in plants and fungi). Here, we present a set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes. This set of guidelines is not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response.

Impact Factor

Scopus SNIP

Web of Science
Times Cited

Altmetric

4.657

1.010

1982