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Friberg, A.R. ; Corsini, L.* ; Mourao, A. ; Sattler, M.

Structure and ligand binding of the extended Tudor domain of D. melanogaster Tudor-SN.

J. Mol. Biol. 387, 921-934 (2009)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
The Tudor-SN protein (p100, SND1) has been implicated in a variety of cellular processes, Such as transcription, processing of edited double-stranded RNA, and splicing regulation. Molecular details of these functions are not vet understood. Tudor domains have previously been shown to bind methylated ligands, such as methylated lysines and arginines. It has been suggested that the role of TUdor-SN in splicing may involve binding to such methylated ligands or to the methylated 5' cap of spliceosomal snRNAs. Here, we report the crystal structure of the extended Tudor domain of Tudor-SN from Drosophila melanogaster to a resolution of 2.1 angstrom. NMR secondary chemical shifts, relaxation data, and residual dipolar Couplings indicate that the solution and Crystal Structures are similar. Binding of various ligands was investigated by NMR. Binding sites and affinities were characterized by chemical shift perturbations. We show that the aromatic cage of the Tudor domain specifically binds a peptide containing symmetrically dimethylated arginines (sbMA) with micromolar affinity, while the same peptide comprising nonmethylated arginines does not show significant chemical shift perturbations. Tudor-SN preferentially recognizes sDMA over asymmetrically dimethylated arginine (aDMA). In contrast, two 5' cap analogues with different methylation patterns, as well as mono-, di-, and trimethyllysines, show no binding. Our data demonstrate that the Tudor domain of Tudor-SN specifically recognizes sDMA-containing ligands. The aromatic cage of TUdor-SN is very similar to the one in the Tudor domain of the survival of motor neuron protein, which also recognizes sDMA peptides, indicating a conserved binding motif for this methylation mark. Recognition of sDMA in the C-terminal tails of spliceosomal Sm proteins suggests how Tudor-SN may interact with small nuclear ribonucleoprotein particles during the regulation of splicing.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Tudor-SN; p-100; NMR; crystallography; sDMA; protein secondary structure; transcriptional coactivator; staphylococcal nuclease; macromolecular structures; nmr experiments; chemical-shift; sm proteins; dna-repair; p100; rna
Sprache
Veröffentlichungsjahr 2009
HGF-Berichtsjahr 2009
ISSN (print) / ISBN 0022-2836
e-ISSN 1089-8638
Zeitschrift Journal of Molecular Biology
Quellenangaben Band: 387, Heft: 4, Seiten: 921-934 Artikelnummer: , Supplement: ,
Verlag Elsevier
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Structural Biology (STB)
POF Topic(s) 30203 - Molecular Targets and Therapies
Forschungsfeld(er) Enabling and Novel Technologies
PSP-Element(e) G-503000-001
PubMed ID 19232356
DOI 10.1016/j.jmb.2009.02.018
Scopus ID 62649142373
Erfassungsdatum 2009-12-31
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